C6490
Anti-phospho-c-Kit (SCFR) (pTyr823) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-phospho-SCF Receptor (pTyr823)
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About This Item
MDL number:
UNSPSC Code:
51111800
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human, canine, mouse, rat
technique(s)
western blot: 0.1-1.0 μg/mL using M07e cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... KIT(3815)
mouse ... Kit(16590)
rat ... Kit(64030)
General description
Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; c-Kit (Stem cell factor receptor, SCF R; CD117) being the most critical. The signalling triggered by c-Kit results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. Tyrosine 823 is a major autophosphorylation site of c-Kit. The secondary pathways activated by c-Kit include Akt, mTOR, MAPK, Sos-Grb2 and c-Jun. In addition to development of mast cells, c-Kit also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF R is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
The antibody detects human c-Kit (145 kDa). Cow and dog (100% homologous), mouse, rat and chicken (92% homologous) have not been tested, but are expected to react.
The antibody detects human c-Kit (145 kDa). Cow and dog (100% homologous), mouse, rat and chicken (92% homologous) have not been tested, but are expected to react.
Immunogen
synthetic phosphopeptide derived from the region of human c-Kit that contains tyrosine 823.
Application
Anti-phospho-c-Kit (SCFR) pTyr823 antibody may be used for immunoblotting at a working concentration of 0.1-1.0 μg/ml.
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dean D Metcalfe
Blood, 112(4), 946-956 (2008-08-08)
Mast cells have been recognized for well over 100 years. With time, human mast cells have been documented to originate from CD34+ cells, and have been implicated in host responses in both innate and acquired immunity. In clinical immunology, they
Mi-Sun Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 180(7), 4586-4595 (2008-03-21)
Little is known about the signals downstream of PI3K which regulate mast cell homeostasis and function following FcepsilonRI aggregation and Kit ligation. In this study, we investigated the role of the mammalian target of rapamycin complex 1 (mTORC1) pathway in
Shoko Iwaki et al.
The Journal of biological chemistry, 280(48), 40261-40270 (2005-09-24)
Stem cell factor (SCF) acts in synergy with antigen to enhance the calcium signal, degranulation, activation of transcription factors, and cytokine production in human mast cells. However, the underlying mechanisms for this synergy remain unclear. Here we show, utilizing bone
M Tsai et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(14), 6382-6386 (1991-07-15)
We investigated the effects of a newly recognized multifunctional growth factor, the c-kit ligand stem cell factor (SCF), on mouse mast cell proliferation and phenotype. Recombinant rat SCF164 (rrSCF164) induced the development of large numbers of dermal mast cells in
Alasdair M Gilfillan et al.
Immunological reviews, 228(1), 149-169 (2009-03-18)
Mast cell mediator release represents a pivotal event in the initiation of inflammatory reactions associated with allergic disorders. These responses follow antigen-mediated aggregation of immunoglobulin E (IgE)-occupied high-affinity receptors for IgE (Fc epsilon RI) on the mast cell surface, a
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