C5973

Monoclonal Anti-CCR-5 antibody produced in mouse

clone 45523, purified immunoglobulin, lyophilized powder

• MDL number: MFCD01864608
• UNSPSC Code: 51111800

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 45523, monoclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): flow cytometry: suitable; immunohistochemistry: 25 μg/mL using paraffin-embedded human lymph node tissue sections
• isotype: IgG2b
• UniProt accession no.: P51681
• storage temp.: −20°C
• Gene Information: human ... CCR5(1234)

Description

General description

CCR-5 is a chemokine receptor belonging to the super family of seven-transmembrane G-protein coupled receptors that mediates cellular functions of immune response, leukocyte migration, development and angiogenesis. CCR-5 is expressed on macrophages and Th1 cells activated on inflammation, osteoclasts and vascular smooth muscle cells. Three CC chemokines, CCL-3, -4 and -5 bind efficiently to CCR-5, whereas CCL-, -8 and -13 bind less efficiently. The expression of CCR-5 protein is most important in resistance to HIV-1 and hepatitis C virus infections and reportedly influences the course and progression of the infection. Monoclonal Anti-CCR-5 antibody specifically recognizes human CCR-5. The antibody shows no cross reactivity with CCR-1, CCR-2, or CCR-3.

Specificity

The antibody reacts with human CCR-5 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2 or CCR-3. This antibody can be used to detect CCR-5 present on stimulated human PBMCs that have been fixed with 2% formaldehyde, but not on unfixed cells.

Immunogen

human CCR-5 transfected NSO mouse myeloma cells.

Application

Monoclonal Anti-CCR-5 antibody may be used for flow cytometry; the antibody may be diluted to 25 μg/ml before adding 10 μL of this dilution to 1-2.5 X 10⁶ cells. The antibody detects CCR-5 present on stimulated human PBMCs (peripheral blood mononuclear cells) only after the cells are fixed with 2% formaldehyde. The antibody does not detect CCR-5 on unfixed human PBMCs. For immunohistochemistry a working concentration of 25 μg/ml using paraffin-embedded human lymph node tissue sections.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Purified using protein G.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)