The concentration of this product varies from lot to lot and may range from 1.0 - 1.2 mg/mL. The lot specific concentration is reported in the batch Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/c5585#product-documentation
C5585
Monoclonal Anti-Biotin–Cy3™ antibody produced in mouse
clone BN-34, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Monoclonal Anti-Biotin
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0.2 ML
$276.50
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0.2 ML
$276.50
About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
$276.50
List Price$395.00Save 30%Web-Only Promotion
Available to ship onApril 29, 2025Details
Request a Bulk Order
Recommended Products
biological source
mouse
Quality Level
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BN-34, monoclonal
form
buffered aqueous solution
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
indirect immunofluorescence: 1:200
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is a naturally occurring vitamin and co-enzyme required by cells in living organisms or in culture.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
Immunogen
Biotin-KLH conjugate
Application
May be substituted for avidin to decrease non-specific binding.
Monoclonal Anti-Biotin−Cy3™ antibody produced in mouse has been used in:
- fluorescent microscopy
- RNA fluorescence in situ hybridization (FISH){36
- immunostaining
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Biochem/physiol Actions
Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Monoclonal Anti-Biotin- Cy3 can be used in many other applications where biotin can be introduced as target label. It can be used in detection of DNA and mRNA by sensitive non-isotopic in situ hybridization. It may also be used for detection of microinjected biotin-haptenized cytoskeletal proteins allowing examination of the pattern of incorporation and turnover of cytoskeletal proteins in living cells.
Physical form
Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Legal Information
Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Jennifer A Ralph et al.
The American journal of pathology, 170(3), 1121-1133 (2007-02-27)
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Park B, et al.
Light: Science & Applications, 3(12), e222-e222 (2014)
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