C4856

Anti-CASK/LIN2 (KA-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD03454854
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~112 kDa
• species reactivity: canine, human
• technique(s): immunoprecipitation (IP): 5-10 μg using lysate of 3x10⁵ cultured MDCK cells; microarray: suitable; western blot (chemiluminescent): 1:300 using whole cell extract of cultured human Jurkat and dog MDCK kidney cells
• UniProt accession no.: O14936
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CASK(8573)

Description

General description

CASK/Lin-2 (calcium/calmodulin-dependent serine kinase) has an N-terminal domain similar to calcium-calmodulin dependent protein kinase (CAM), a Lin-7 binding domain, a single PDZ domain, an SH3 domain, and a C-terminal GUK (guanylate kinase) homologous domain. CASK/Lin-2 gene is mapped to human chromosome Xp11.4.

Calcium/Calmodulin-dependent Serine Kinase (CASK/Lin-2) is predominant in the brain, may be ubiquitously expressed. It localizes to the lateral and/or basal plasma membrane regions in epithelial cells.

Specificity

Anti-CASK/Lin-2 specifically recognizes humanCASK/Lin-2.

Immunogen

synthetic peptide corresponding to human CASK/LIN-2 sequence, (amino acids 393-409) conjugated to KLH.

Application

Anti-CASK/LIN2 (KA-17) antibody produced in rabbit is suitable for:

• immunoprecipitation at a concentraqtion of 5-10μg, using lysate of 3x105 cultured MDCK cells
• western blot (chemiluminescent) at a dilution of 1:300, using whole cell extract of cultured human Jurkat and dog MDCK kidney cells

Biochem/physiol Actions

CASK/Lin-2 (Calcium/Calmodulin-dependent Serine Kinase) is translocated into the nucleus to regulate gene expression. It interacts via its PDZ domain with the cytosolic tail of the transmembrane cell surface heparin sulfate proteoglycan Syndecan-2. In neurons it interacts with neurexins. In addition, CASK/Lin-2 is capable of interacting with the actinbinding band 1 protein, neuronal munc18-1-interacting protein 1(Mint-1/Lin-10), protein lin-7 homolog A (Veli/Lin-7) proteins, junctional adhesion molecule (JAM), disks large homolog 1 (hDlg), and the nuclear T-box brain transcription factor 1 (Tbr-1).

Mutations in the CASK gene is implicated in the X-linked brain malformation syndrome. A missense mutation in the CASK gene is also reported in the pathophysiology of FG syndrome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.