C4839
Cytochrome P450 Reductase from rabbit liver induced with phenobarbital
buffered aqueous glycerol solution
Synonym(s):
NADPH:Ferrihemoprotein oxidoreductase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12161501
NACRES:
NA.47
Recommended Products
assay
~90% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
15-45 units/mg protein (Bradford)
mol wt
~80 kDa
shipped in
wet ice
storage temp.
−20°C
Biochem/physiol Actions
The enzyme catalyses electron transfer to cytochrome P450. This system plays a major role in detoxification of drugs and xenobiotics, activation of carcinogens, and metabolism of endogenous substrates such as steroids.
Packaging
Package size based on protein content.
Unit Definition
One unit will catalyze the reduction of 1.0 μmole of cytochrome c by NADPH per min at pH 7.7 at 30 °C.
Physical form
Solution in 30 mM potassium phosphate buffer, pH 7.7, and 0.1 mM EDTA containing 50%(v/v) glycerol.
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Backes, W.L. et al.
Cytochrome P450, 15-34 (1993)
E A Shephard et al.
Analytical biochemistry, 129(2), 430-433 (1983-03-01)
NADPH-cytochrome c (P-450) reductase from liver microsomes of phenobarbital-treated rats has been purified in a single step by affinity chromatography on agarose-hexane-adenosine 2',5'-diphosphate. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, enzyme assay, and radioimmunoassay the protein obtained by this
Hsia-lien Lin et al.
Chemical research in toxicology, 25(12), 2642-2653 (2012-09-29)
The reaction of peroxynitrite (PN) with purified human cytochrome P450 3A4 (CYP3A4) resulted in the loss of the reduced-CO difference spectrum, but the absolute absorption spectrum of the heme was not significantly altered. The loss of 7-benzyloxy-4-(trifluoromethyl)coumarin (BFC) O-debenzylation activity
Lei Hu et al.
Pharmacogenetics and genomics, 22(11), 812-819 (2012-10-11)
The redox reaction of cytochrome P450 enzymes (CYP) is an important physiological and biochemical reaction in the human body, as it is involved in the oxidative metabolism of both endogenous and exogenous substrates. Cytochrome P450 oxidoreductase (POR) is the only
Q Shen et al.
Genetics and molecular research : GMR, 11(3), 3298-3309 (2012-10-20)
Finding an efficient and affordable treatment against malaria is still a challenge for medicine. Artemisinin is an effective anti-malarial drug isolated from Artemisia annua. However, the artemisinin content of A. annua is very low. We used transgenic technology to increase
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