C4491

Anti-Calcium-Sensing Receptor antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-CASR
• MDL number: MFCD02262635
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• availability: not available in Japan
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 4-8 μg/mL using human parathroid gland tissue
• UniProt accession no.: P41180
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CASR(846) ; mouse ... Casr(12374) ; rat ... Casr(24247)

Description

General description

The CASR (calcium sensing receptor) gene is mapped to human chromosome 3q13.33-q21.1. It is ubiquitously expressed and codes for protein containing 1,078 amino acid residues. The protein structure is divided into three domains : a large, extracellular domain at the amino terminal, a transmembrane domain and an intracellular carboxyl domain.

Immunogen

synthetic peptide corresponding to the extracellular N-terminal domain of human calcium-sensing receptor, conjugated to KLH. The immunizing peptide has 100% homology with the rat and mouse gene.

Application

Anti-calcium-sensing receptor antibody produced in rabbit is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 4-8 μg/mL using human parathyroid gland tissue.

Biochem/physiol Actions

CASR (calcium sensing receptor) is an integral membrane protein. It senses changes in the extracellular concentration of calcium ions and maintains its homeostasis within the system. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. The antibody specifically recognizes human calcium sensing receptor by immunohistochemistry with formalin-fixed, paraffin-embedded tissues. Calcium sensing receptor is actively involved in the secretion of peptide hormones such as parathyroid hormone, corticotropin hormone, adrenocorticotropic hormone, gastrin, insulin and growth hormone. It is associated with the function of K⁺, Ca²⁺ and water ion channels. Regulation of pituitary tumor-transforming gene expression is controlled by CasR. CasR is associated with cell proliferation (colonic/ovarian surface epithelial cells, testis Leydig cell cancer cells, fibroblasts, and keratinocytes) and cell differentiation (keratinocytes, goblet cells and mammary epithelial cells) processes. It mediates chemotaxis of pre osteoblastic cells and macrophages. It regulates apoptosis in cancer cells including Leydig cell tumour and prostate cancer cell line.

Physical form

Solution in phosphate buffered saline, pH 7.7, containing 0.01% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.