C4358
CD152/Fc Chimera, Non-cytolytic from mouse
≥98% (SDS-PAGE), recombinant, expressed in NS.1 cells, lyophilized
Synonym(s):
CTLA4/Fc Chimera, non-cytolytic
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
recombinant
expressed in NS.1 cells
assay
≥98% (SDS-PAGE)
form
lyophilized
mol wt
97 kDa
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Ctla4(12477)
General description
The extracellular domain (160 amino acids) of mouse CD152 is fused to mouse IgG2a Fc domain in which mutations to the complement (C1q) and FcγR I binding sites of the Fcγ2a fragment render the Fc fusion protein incapable of direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
Biochem/physiol Actions
CD152 (CTLA4), a cell surface glycoprotein expressed at low levels on activated T cells, is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). A related cell surface glycoprotein, CD28, binds to CD80 and CD86 with lower affinity. The soluble CD152/Fc chimeric fusion protein blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.
High affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2); blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.
Physical form
Lyophilized from solution of 0.2 μm filtered, in phosphate buffered saline, pH 7.4, with no preservative added.
Preparation Note
Purified from serum-free tissue culture supernatant
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
W Steurer et al.
Journal of immunology (Baltimore, Md. : 1950), 155(3), 1165-1174 (1995-08-01)
To test the hypothesis that blockade of B7-triggered costimulation by donor cells could preclude allograft rejection, we coated crude islet allograft preparations in vitro for 1 h with a murine CTLA4/Fc fusion protein. Murine CTLA4/Fc blocks the proliferative response in
P S Linsley et al.
The Journal of experimental medicine, 174(3), 561-569 (1991-09-01)
Functional interactions between T and B lymphocytes are necessary for optimal activation of an immune response. Recently, the T lymphocyte receptor CD28 was shown to bind the B7 counter-receptor on activated B lymphocytes, and subsequently to costimulate interleukin 2 production
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
