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C4187

Sigma-Aldrich

Cyanoborohydride Coupling Buffer

Synonym(s):

Coupling buffer

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500 ML
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500 ML
$262.65
1 L
$477.00

About This Item

UNSPSC Code:
12161700
NACRES:
NA.56

$262.65

List Price$309.00Save 15%
Web-Only Promotion

Available to ship onApril 29, 2025Details

form

liquid

Quality Level

200

reaction suitability

reagent type: reductant

technique(s)

affinity chromatography: suitable

application(s)

life science and biopharma

storage temp.

2-8°C

Application

A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond.
Cyanoborohydride Coupling Buffer has been used:
  • in coupling reactions between amines and glutaraldehyde[1]
  • to reduce hydrazone bond to a stable hydrazide bond[2]
  • as a component in oligonucleotide reaction mixture for coverslips functionalization[3]

Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.

Biochem/physiol Actions

Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines.[4][5] It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support.[6]

Preparation Note

0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

hcodes

H302,H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000434139
0000434139
0000378841
0000378841
0000308220

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Hai-mei Hu et al.
Journal of biomaterials science. Polymer edition, 23(5), 677-695 (2011-03-08)
A non-viral gene-delivery system has been used to deliver plasmid DNA into specific cell types because of its safety and ease of manufacture. Receptor-mediated gene transfer is currently a promising gene-delivery technique. To specifically target genes to asialoglycoprotein receptor of
Handbook of Affinity Chromatography (2005)
Polymer-supported triacetoxyborohydride: a novel reagent of choice for reductive amination
Bhattacharyya S, et al.
Tetrahedron Letters, 44(27), 4957-4960 (2003)
V S Hornsey et al.
Journal of immunological methods, 93(1), 83-88 (1986-10-23)
For coupling proteins to Sephacryl gels periodate oxidation of these gels was investigated as an alternative method to cyanogen bromide activation. Optimum conditions were studied with respect to periodate concentration, time of oxidation, pH and type of coupling buffer, concentration
Binding between the integrin ?X?2 (CD11c/CD18) and heparin.
Vorup-Jensen T, et al.
Test, 282(42), 30869-30877 (2007)
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