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Key Documents

C4118

Sigma-Aldrich

Anti-CRMP1 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Collapsin Response Mediator Protein 1, Anti-Dihydropiraminidase-like 1 (DPYSL1), Anti-Dihydropyriminidase Related Proterin-1 (DRP1, DRP-1)

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~62 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

western blot: 0.5-1 μg/mL using HEK-293T cells expressing human CRMP1, mouse brain, and rat brain extracts (S1 fraction)

UniProt accession no.

Q14194

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CRMP1(1400)
mouse ... Crmp1(12933)
rat ... Crmp1(25415)

General description

Collapsin response mediator proteins-1 (CRMP1) belongs to CRMP family of proteins. The protein localizes in the cytoplasm, at the centrosomes, with the mitotic spindle and midbody depending upon the cell cycle stage.

Immunogen

synthetic peptide corresponding to amino acids 174-190 of human CRMP1, conjugated to KLH. This sequence is identical in mouse and rat CRMP1.

Application

Anti-CRMP1 (N-terminal) antibody is suitable of western blot at a concentration of 0.5-1μg/mL using HEK-293T cells expressing human CRMP1, mouse brain, and rat brain extracts (S1 fraction). Anti-CRMP1 (N-terminal) specifically recognizes human, rat, and mouse CRMP1 by immunoblotting, ∼62 kDa.

Biochem/physiol Actions

Collapsin response mediator proteins (CRMP) family proteins are cytosolic phosphoproteins which are expressed in the nervous system and involved in neuronal differentiation and axonal guidance. CRMP1 is important for regulating axon outgrowth in Semaphorin 3A (SEMA3A) signaling pathways. Changes in CRMP1 activity results in cytokinesis defect. CRMP1 is also an invasion-suppressor gene and is linked with cancer invasion and metastasis. It is down-regulated in lung adenocarcinoma cells via nuclear factor-κB p50. In a rat model, CRMP1 is associated with development of temporal lobe epilepsy (TLE).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
077K4873

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Li Yao et al.
Molecular neurobiology, 53(2), 879-893 (2014-12-21)
CRMP1, a member of the collapsin response mediator protein family (CRMPs), was reported to regulate axon outgrowth in Sema3A signaling pathways via interactions with its co-receptor protein neuropilin-1 and plexin-As through the Fyn-cyclin-dependent kinase 5 (CDK5) cascade and the sequential
Jing Luo et al.
Neurochemical research, 37(7), 1381-1391 (2012-02-24)
The Collapsin Response Mediator Protein-1 (CRMP-1) is a brain specific protein identified as a signaling molecule of Semaphorin-3A and act as axon repellent guidance factor in nervous system. Recent studies indicated that axon guidance molecules may play a role in
Ming Gao et al.
Biochemical and biophysical research communications, 376(2), 283-287 (2008-09-11)
Lung adenocarcinoma Cl1-5 cells were selected from parental Cl1-0 cells based on their high metastatic potential. In a previous study, CRMP-1, an invasion suppressor gene, was shown to be suppressed in Cl1-5 cells. However, the regulation of CRMP-1 expression has
Tzu-Chi Chen et al.
Journal of proteome research, 8(11), 4943-4953 (2009-10-06)
Midbody, a transient organelle-like structure, is known as central for abscission and is indispensable for termination of cytokinesis. Here, we used the midbody proteome inventories to construct the potential midbody protein-protein interaction (PPI) network. To delineate novel regulators participating in
J Y Shih et al.
Journal of the National Cancer Institute, 93(18), 1392-1400 (2001-09-20)
Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL(1-0), CL(1-1), CL(1-5), and CL(1-5)-F(4) in order of increasing invasive activity) for such
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