C3835
Ciliary Neurotrophic Factor from rat
recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE), carrier free
Synonym(s):
CNTF
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About This Item
MDL number:
UNSPSC Code:
51111800
Recommended Products
biological source
rat
recombinant
expressed in E. coli
assay
≥98% (SDS-PAGE)
form
lyophilized powder
mol wt
22.7 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white
solubility
water: soluble 0.10 mL, clear to slightly hazy, colorless
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... Cntf(25707)
Biochem/physiol Actions
CNTF prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation; most of its known actions are restricted to cells of the nervous system.
Ciliary neurotrophic factor was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat ciliary neurotrophic factor share ~83% sequence homology and show cross-reactivity in bioactivity.
Physical form
Lyophilized from a 0.2 μm filtered solution of 5 mM sodium acetate, pH 6.5.
Analysis Note
The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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