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C3777

Sigma-Aldrich

Casein fluorescein isothiocyanate from bovine milk

Type II, essentially salt-free, lyophilized powder

Synonym(s):

FITC-casein

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25 MG
$196.00
100 MG
$594.00

$196.00

Available to ship onApril 29, 2025Details

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25 MG
$196.00
100 MG
$594.00

About This Item

MDL number:
MFCD00130743
UNSPSC Code:
12352204
NACRES:
NA.32

$196.00

Available to ship onApril 29, 2025Details

type

Type II

Quality Level

200

form

essentially salt-free, lyophilized powder

extent of labeling

20-50 μg FITC per mg solid

solubility

water: 5 mg/mL, clear to hazy, yellow to orange

storage temp.

2-8°C

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General description

Casein is a major milk phospho-protein and constitutes about 80% of the total protein content in milk. Bovine milk is composed of four types of casein such as, αS1, αS2, β, and κ.[1][2] Casein exists as micelles in milk.[2]

Application

Casein fluorescein isothiocyanate from bovine milk has been used:
  • as a substrate for semi-quantitative analysis of protease in Arabidopsis cells[3]
  • to determine the caseinolytic activity of secreted LasB (elastase), alkaline protease and protease IV[4]
  • for determining the SpeB (cysteine protease) proteolytic activity in Streptococcus pyogenes cells[5]

highly sensitive protease substrate

Biochem/physiol Actions

Casein micelle system is involved in the prevention of pathological calcification of mammary glands.[2]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000430893
0000430893
0000411339
0000411339
0000391256

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Thomas S Murray et al.
Journal of medical microbiology, 59(Pt 5), 511-520 (2010-01-23)
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen capable of acutely infecting or persistently colonizing susceptible hosts. P. aeruginosa colonizes surfaces in vitro by either biofilm formation or swarming motility. The choice of behaviour is influenced by the physical properties of
Cationic antimicrobial peptides disrupt the Streptococcus pyogenes ExPortal
Vega LA and Caparon MG
Molecular Microbiology, 85(6), 1119-1132 (2012)
Casein and Whey Proteins in Human Health
Milk and Dairy Products as Functional Foods, 102(6), 94-146 (2014)
Invited review: Understanding the behavior of caseins in milk concentrates
Corredig M, et al.
Journal of Dairy Science, 102(6), 4772-4782 (2019)
Swarming motility, secretion of type 3 effectors and biofilm formation phenotypes exhibited within a large cohort of Pseudomonas aeruginosa clinical isolates
Murray T S, et al.
Journal of Medical Microbiology, 59(Pt 5), 511-511 (2010)
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