C3114

Anti-Calpain-13 (Domain III, N-Terminal), Large Subunit antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

• MDL number: MFCD05665191
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: rat, human, mouse
• concentration: ~1 mg/mL
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1:1,000
• UniProt accession no.: Q6MZZ7
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CAPN13(92291) ; mouse ... Capn13(381122) ; rat ... Capn13(362701)

Description

General description

Calpains are calcium-activated, non-lysosomal cysteine proteases that cleave cytoskeletal and submembranous proteins. It is an intracellular cysteine protease that is present in all mammalian tissues. Anti-Calpain-13 (Domain I, N-Terminal), Large Subunit is developed in rabbit using a synthetic peptide corresponding to domain I of the large subunit of human calpain 13 (capn-13) as immunogen. The antibody is affinity purified using agarose to which the immunogen peptide has been bound.

Immunogen

synthetic peptide corresponding to domain III of the large subunit of human calpain 13.

Application

Anti-Calpain-13 is suitable for immunohistochemistry, immunoprecipitation and indirect ELISA. It is also suitable for western blot at a dilution of 1:1,000

Biochem/physiol Actions

Calpains are involved in a variety of processes including cytoskeletal reorganization, muscle protein degradation, cell proliferation, differentiation, and vesicular secretion. Anti-Calpain-13 (Domain I, N-Terminal), Large Subunit recognizes domain I in the large subunit of human calpain 13 by various immunochemical techniques including immunoblotting, immunoprecipitation, immunohistochemistry, and ELISA. It detects human, rat, and mouse calpain 13 and does not crossreact with other calpain family members (calpain 1, calpain 2, calpain 3, etc.). The antibody binds to the reduced and non-reduced protein. By immunoblotting against the reduced protein, the antibody identifies bands at approximately 64 kDa, 52 kDa, 48 kDa, 40 kDa, and aseries of smaller forms.

Physical form

Solution in 0.01 M phosphate buffered saline containing 50% glycerol and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids