C3104
Anti-CD30 antibody produced in goat
affinity isolated antibody, lyophilized powder
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About This Item
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
mouse
technique(s)
indirect ELISA: 0.5-1.0 μg/mL
western blot: 0.1-0.2 μg/mL
UniProt accession no.
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Tnfrsf8(21941)
General description
CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. The extracellular domain of recombinant mouse CD30/Fc is fused to the carboxyterminal 6X histidine-tagged Fc portion of human IgG1 by a polypeptide linker.
Specificity
Shows no cross-reactivity with recombinant human CD30. The antibody will stimulate the bioactivity of mouse splenic B cell proliferation in the presence of IL-4 and IL-5.
Immunogen
recombinant mouse CD30, extracellular domain, expressed in NSO cells.
Application
Anti-CD30 is suitable for western blotting at a concentration of 0.1-0.2μg/mL and indirect ELISA at a concentration of 0.5-1.0μg/mL
Biochem/physiol Actions
Anti-CD30 recognizes recombinant mouse CD30 by immunoblotting and ELISA. In immunoblotting and ELISA, this antibody does not cross-react with recombinant human CD30. Reduced mouse CD30/Fc monomer has a calculated molecular mass of approximately 55 kDa. Due to glycosylation, the recombinant protein migrates as an approximately 90 -100 kDa protein in SDS-PAGE under reducing conditions. CD30 specific IgG is purified from goat serum using mouse CD30 affinity chromatography. CD30 has a critical role in the pathophysiology of Hodgkin′s disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. Anti-CD30 is developed in goat using purified recombinant mouse CD30 extracellular domain, expressed in mouse NSO cells as immunogen.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline
Reconstitution
Reconstitute with 1 mL sterile PBS to yield 0.1 mg/ml solution.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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M A Bowen et al.
Journal of immunology (Baltimore, Md. : 1950), 156(2), 442-449 (1996-01-15)
Murine CD30 cDNA predicts a protein of 498 amino acids with homology to the TNF receptor family of proteins characterized by repeated cysteine-rich motifs in the extracellular domain. Murine CD30, although homologous to human CD30, has a 90 amino acid
R Chiarle et al.
Journal of immunology (Baltimore, Md. : 1950), 163(1), 194-205 (1999-06-29)
The biological function of CD30 in the thymus has been only partially elucidated, although recent data indicate that it may be involved in negative selection. Because CD30 is expressed only by a small subpopulation of medullary thymocytes, we generated transgenic
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