C2872

Anti-CSTF1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Cleavage stimulation factor, 3-prime PRE-RNA, subunit 1, 50-KD, Anti-CstF-50, Anti-CstFp50
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~48 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using K562 cell lysates; western blot: 1-2 μg/mL using HepG2 cell lysates
• UniProt accession no.: Q05048
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CSTF1(1477) ; mouse ... Cstf1(67337) ; rat ... Cstf1(311670)

Description

General description

Cleavage stimulation factor (CSTF) is a heterotrimer consisting of subunits of 50, 64 and 77 kDa referred to as CSTF1, CSTF2 and CSTF3 respectively. Anti-CSTF1 (N-terminal) is produced in rabbit using a synthetic peptide as immunogen. It corresponds to amino acids 2-16 of human CSTF1 conjugated to KLH. The corresponding sequence is identical in mouse and rat. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Application

Anti-CSTF1 is used in immunoprecipitation at a concentration of 5-10 μg using K562 cell lysates. The antibody may be used in several immunochemical techniques including immunoblotting (∼48 kDa).

Biochem/physiol Actions

CSTF1 is required for in vitro cleavage activity. CSTF1 also interacts directly with the RNA polymerase II CTD, suggesting a role in linking transcription and mRNA 3′-ends processing. It is required for polyadenylation and 3′-end cleavage of mammalian pre-mRNAs.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.