PromoCell’s human pericytes derived from microvessels of the human placenta are cryopreserved at the end of secondary culture (P1) and after thawing are considered P2. The number of populations doublings is not determined for each individual cell lot, however they are guarenteed to be grown for at least 15 population doublings.
C-28040
Pericyte Growth Medium
Ready-to-use kit including Basal Medium and SupplementMix, 500 ml
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About This Item
UNSPSC Code:
12352207
Recommended Products
form
aqueous solution
packaging
pkg of 500 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
General description
Pericyte Growth Medium (Ready-to-use)
Application
Pericyte Growth Medium has been developed for the in vitro cultivation of human pericytes from all tissues. In standard culture media pericytes tend to grow slowly and undergo early senescence, whereas in PromoCell Pericyte Growth Medium the cells are optimally supported for cell proliferation and maintenance of multipotency.Recommended for:Human Pericytes
Quality
All lots of PromoCell Pericyte Growth Medium are subjected to comprehensive quality control tests using primary human placenta-derived pericytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested pericytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Storage and Stability
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Subculture Routine
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Storage Class
10 - Combustible liquids
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Articles
What are pericytes? What are common pericyte markers? Are pericytes mesenchymal stem cells? Learn more about pericytes in our FAQ page. Explore over 350 PromoCell products.
Primary human pericytes and cell culture media optimized for the in vitro cultivation of human pericytes from all tissues. Protocols for handling, cell passaging, media, and product use.
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How long do pericytes grow in culture?
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What markers are used to characterize pericytes?
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Pericytes are positive for CD146, PDGFRβ, αSMA, CD13, NG2, Desmin, and negative for CD31, CD34, CD45, and CD56.
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How are pericytes isolated?
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Pericyte isolation generally starts with enzymatic digestion of tissue with Trypsin EDTA, usually followed by the isolation of microvessel fragments via successive filtration steps. Pure pericyte cultures can be obtained through either positive or negative immuno-selection via the use of magnetic beads or flow cytometry using the antibody markers above.
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