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C-26502

Sigma-Aldrich

Follicle Dermal Papilla Cell Growth Medium

Kit including Basal Medium and SupplementPack, 500 ml

Synonym(s):

Cell Growth Medium, Dermal Papilla Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

packaging

pkg of 500 ml

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

General description

Follicle Dermal Papilla Cell Growth Medium Kit

Application

PromoCell Follicle Dermal Papilla Cell Growth Medium is a low-serum medium that has been developed for the in vitro cultivation of Human Follicle Dermal Papilla Cells. The medium is optimized for primary cultures of fetal, neonatal, and adult tissue, or for the maintenance of follicle dermal papilla cells.Recommended for:Human Follicle Dermal Papilla Cells

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Follicle Dermal Papilla Cell Growth Medium are subjected to comprehensive quality control tests using primary human follicledermal papilla cells. Each lot is checked for growth promoting activity, adherence rate and typical morphology of the tested follicle dermal papilla cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.04 ml / mlBovine Pituitary Extract: 0.004 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Storage and Stability

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

Storage Class

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.

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