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Key Documents

C2622

Sigma-Aldrich

Anti-CPSF1 (N-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CPSF160, Anti-Cleavage and polyadenylation specific factor 1, 160kDa, Anti-HSU37012, Anti-P/cl.18

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~160 kDa

species reactivity

human

concentration

~1.5 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysate of HEK-293T cells over expressing human CPSF1

UniProt accession no.

Q10570

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CPSF1(29894)

Application

Anti-CPSF1 (N-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μg using lysate of HEK-293T cells over expressing human CPSF1.

Biochem/physiol Actions

Cleavage and polyadenylation specificity factor subunit 1 is a protein encoded by the CPSF1 gene in humans. CPSF1 is the largest subunit of the CPSF complex composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1 and is located in the nucleus. CPSF is a multi-subunit protein that plays a central role in 3′ processing of mammalian pre-mRNAs. CPF interacts directly with the AAUAAA recognition signal in the RNA and is required for the cleavage as well as for polyadenylation reaction during 3′ processing of messenger RNA precursors.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Certificates of Analysis (COA)

Lot/Batch Number
079K4755

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Qin Yang et al.
Wiley interdisciplinary reviews. RNA, 2(5), 732-747 (2011-08-09)
3' processing is an essential step in the maturation of all messenger RNAs (mRNAs) and is a tightly coupled two-step reaction: endonucleolytic cleavage at the poly(A) site is followed by the addition of a poly(A) tail, except for metazoan histone
K G Murthy et al.
Genes & development, 9(21), 2672-2683 (1995-11-01)
Cleavage-polyadenylation specificity factor (CPSF) is a multisubunit protein that plays a central role in 3' processing of mammalian pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.
W Keller et al.
The EMBO journal, 10(13), 4241-4249 (1991-12-01)
Cleavage and polyadenylation factor (CPF) is required for the cleavage as well as for the subsequent polyadenylation reaction during 3' processing of messenger RNA precursors. Here, we have investigated the interaction of CPF and poly(A) polymerase with short RNA substrates.

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