C2372
Anti-CSTF1 (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Cleavage stimulation factor, 3-prime PRE-RNA, subunit 1, 50-KD, Anti-CstF-50, Anti-CstFp50
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200 μL
$562.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~48 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using K562 lysates
western blot: 2-4 μg/mL using K562 lysates
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CSTF1(1477)
mouse ... Cstf1(67337)
rat ... Cstf1(311670)
General description
Cleavage stimulation factor 1 (CSTF1) contains seven WD-40 repeats. The CSTF1 gene is mapped to human chromosome 20q13.2-q13.31.
Specificity
Anti-CSTF1 (C-terminal) specifically recognizes human CSTF1 (also known as Cleavage stimulation factor).
Application
Anti-CSTF1 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μg using K562 lysates and western blotting at a concentration of 2-4μg/mL using K562 lysates.
Biochem/physiol Actions
CSTF1 is required for in vitro cleavage activity. The WD-40 repeats are crucial for the interaction with CSTF3. CSTF1 also interacts directly with the RNA polymerase II CTD, suggesting a role in linking transcription and mRNA 3′-ends processing.
Cleavage stimulation factor (50kDa) subunit is a protein encoded by the CSTF1 gene in humans. It is one of the multiple factors that plays a significant role during polyadenylation of mammalian pre-mRNAs. The mRNA polyadenylation factor CstF interacts with the BRCA1-associated protein BARD1 resulting in repression of the nuclear mRNA polyadenylation machinery. It participates in the regulation of endogenous transcripts during DNA-damage.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Shea J Andrews et al.
The Lancet. Neurology, 19(4), 326-335 (2020-01-28)
Alzheimer's disease is a debilitating and highly heritable neurological condition. As such, genetic studies have sought to understand the genetic architecture of Alzheimer's disease since the 1990s, with successively larger genome-wide association studies (GWAS) and meta-analyses. These studies started with
F E Kleiman et al.
Cell, 104(5), 743-753 (2001-03-21)
The mRNA polyadenylation factor CstF interacts with the BRCA1-associated protein BARD1, and this interaction represses the nuclear mRNA polyadenylation machinery in vitro. Given the suspected role of BRCA1/BARD1 in DNA repair, we tested whether inhibition of mRNA processing is linked
Y Takagaki et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(4), 1403-1407 (1992-02-15)
Cleavage stimulation factor is one of the multiple factors required for 3'-end cleavage of mammalian pre-mRNAs. We have shown previously that this factor is composed of three subunits with estimated molecular masses of 77, 64, and 50 kDa and that
Murat A Cevher et al.
The EMBO journal, 29(10), 1674-1687 (2010-04-10)
We previously showed that mRNA 3' end cleavage reaction in cell extracts is strongly but transiently inhibited under DNA-damaging conditions. The cleavage stimulation factor-50 (CstF-50) has a role in this response, providing a link between transcription-coupled RNA processing and DNA
Y Takagaki et al.
The Journal of biological chemistry, 267(33), 23471-23474 (1992-11-25)
Cleavage stimulation factor (CstF) is one of the multiple factors required for polyadenylation of mammalian pre-mRNAs in vitro. We have shown previously that this factor is composed of three distinct subunits of 77, 64, and 50 kDa, and that the
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