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C1872

Sigma-Aldrich

Anti-CPSF2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-100-KD subunit, Anti-CPSF100, Anti-Cleavage and polyadenylation specificity factor 2

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

form

buffered aqueous solution

mol wt

antigen ~100 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2-5 μg using A20 cell lysates
western blot: 2-4 μg/mL using K562 cell lysates

UniProt accession no.

Q9P2I0

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CPSF2(53981)
mouse ... Cpsf2(51786)

Immunogen

synthetic peptide corresponding to amino acids 718-732 of human CPSF2 conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-CPSF2 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 2-5μg using A20 cell lysates and western blotting at a concentration of 2-4μg/mL using K562 cell lysates.

Biochem/physiol Actions

Cleavage and polyadenylation specificity factor subunit 2 is a protein encoded by the CPSF2 gene in humans. It is also referred to as CPSF100. CPSF and cleavage stimulation factor (CstF) play an important role during the formation of the 3′ ends of mRNA for 3′ cleavage of the transcript as well as for subsequent polyadenylation. CPSF and poly(A) polymerase (PAP) activity is controlled by Eg2-catalyzed phosphorylation during formation of cytoplasmic polyadenylation complex.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

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A Jenny et al.
Molecular and cellular biology, 14(12), 8183-8190 (1994-12-01)
During the formation of the 3' ends of mRNA, the cleavage and polyadenylation specificity factor (CPSF) is required for 3' cleavage of the transcript as well as for subsequent polyadenylation. Using peptide sequences from a tryptic digest, we have cloned
W Schul et al.
The EMBO journal, 15(11), 2883-2892 (1996-06-03)
The cleavage stimulation factor (CstF), and the cleavage and polyadenylation specificity factor (CPSF) are necessary for 3'-terminal processing of polyadenylated mRNAs. To study the distribution of 3' cleavage factors in the nuclei of human T24 cells, monoclonal antibodies against the
R Hodgman et al.
Development (Cambridge, England), 128(14), 2815-2822 (2001-08-30)
In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB, a sequence-specific RNA binding

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