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Key Documents

C1655

Sigma-Aldrich

Cholera Toxin B subunit

FITC conjugate, lyophilized powder

Synonym(s):

CTB

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250 μG
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250 μG
$136.80
0.5 MG
$262.00

About This Item

MDL number:
MFCD02356522
UNSPSC Code:
12352200
PubChem Substance ID:
329774902
NACRES:
NA.77

$136.80

List Price$152.00Save 10%
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Available to ship onMay 05, 2025Details

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conjugate

FITC conjugate

Quality Level

200

form

lyophilized powder

mol wt

~12 kDa

composition

Protein, ~20% Lowry

extent of labeling

~1.0 mol FITC per mol protein

storage temp.

2-8°C

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

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Application

Cholera Toxin B subunit-fluorescein isothiocyanate (FITC) conjugate has been used as a fluorescent tracer:
  • to label distal cerebrospinal fluid-contacting neurons (dCSF-CNs) in lateral ventricles of rat[1]
  • to stain epididymal sperms of mice[2]
  • to stain peritoneal macrophages of mice[3]

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide

Analysis Note

Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H332,H412

Hazard Classifications

Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000420470
0000420470
0000216592
0000312714
0000327290

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Lindsey C Felix et al.
Aquatic toxicology (Amsterdam, Netherlands), 192, 58-68 (2017-09-18)
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