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Key Documents

C0978

Sigma-Aldrich

Anti-Crk-L antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD01633275
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 38 kDa

species reactivity

mouse, human

technique(s)

immunoprecipitation (IP): 5 μg using 20 μg of a human A431 cell lysate
microarray: suitable
western blot: 1:1,000-1:5,000 using using human A431 and human Raji cell lysate, respectively

UniProt accession no.

P46109

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CRKL(1399)
mouse ... Crkl(12929)

General description

CrkL is a 39kD adaptor protein plays a crucial role in the early steps of IL-2 signal transduction. It also facilitates signaling of β-integrin, B-cell or T-cell receptors. Anti-Crk-L antibody can be used in immunoprecipitation for detecting and localizing CrkL. Rabbit anti-Crk-L antibody reacts specifically with CrkL (38 kD) of human.

Immunogen

A synthetic peptide corresponding to amino acids 283-303 of human Crk-L, conjugated to KLH. The sequence is highly conserved in mouse Crk-L (single amino acid substitution).

Application

Anti-Crk-L antibody can be used in western blotting (diluted 1: 1000, 1:5000) using human epidermal carcinoma A431 cell line and Burkitt lymphoma Raji cell line.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
050K4803

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F Gesbert et al.
The Journal of biological chemistry, 273(7), 3986-3993 (1998-03-28)
Interleukin (IL)-2, a major growth and differentiation factor for T lymphocytes, was found to induce tyrosine phosphorylation of the proto-oncogene products p120-Cbl and CrkL in IL-2-dependent cell lines. We established that, in unstimulated lymphocytes, the Src homology 2 (SH2) and
M Sattler et al.
Leukemia, 12(5), 637-644 (1998-05-21)
CRKL is a 39 kDa adapter protein, originally cloned in proximity to the BCR gene on chromosome 22, which has a key regulatory role in hematopoietic cells. CRKL has one SH2 and two SH3 domains, with 60% homology to CRK

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