C0853

Anti-Crk-II antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD02096152
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 38 kDa
• species reactivity: human, mouse, chicken
• technique(s): microarray: suitable; western blot: 1:3,000 using whole extract of the Burkitt lymphoma Raji cell line; western blot: 1:5,000 using whole extract of chicken fibroblasts
• UniProt accession no.: P46108
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CRK(1398) ; mouse ... Crk(12928)

Description

General description

Crk proteins belong to a family of adaptor proteins with distinct Src homology (SH) domains, SH2 and SH3. Crk-I, Crk-II and CrkL are the cellular homologs of oncogene v-Crk of avian sarcoma virus. While Crk-I and Crk-II are the products of alternate gene splicing, Crk-L is encoded by a different gene although it shares 60% homology with Crk-II. The Crk proteins are involved in many signal transduction pathways. The SH2 domain of Crk proteins interact with several tyrosine kinases such as EGFR, Cbl, p130^cas, paxillin and IRS-1. In humans, both SH2 and SH3 domains of Crk proteins are essential for neuronal differentiation of PC-12 cells, play active role in NGF and p21ras signalling.
Anti-Crk-II specifically recognizes human and chicken Crk-II (38 kDa).

Immunogen

synthetic peptide K-THVRLLDQQNPDEDFS corresponding to the C-terminal of human Crk-II (amino acids 289-304 with N-terminally added lysine), conjugated to KLH. The sequence is identical in human, mouse and chicken Crk-II and highly conserved (single amino acid substitution) in rat Crk-II. This sequence is absent in Crk-I isoform and has limited homology (60%) to Crk-L..

Application

Anti-Crk-II antibody may be used at a working dilution of 1:5000 in whole extract of chicken fibroblasts. It may be used for detection of Crk-II in whole extract of Raji cell line (Burkitt lymphoma). It is also suitable for protein microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.