C0646
Cytodex® 1 microcarrier beads
Cytodex 1, 60-87 μm
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About This Item
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material
dextran beads
sterility
non-sterile
particle size
60-87 μm
density
1.03 g/cm3 at 25 °C
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General description
A general-purpose microcarrier for most established, anchorage-dependent cell lines.
High yield culture of anchorage-dependent cells: Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few mL to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.
Suitable for most established cell lines: Formed by substituting a cross-linked dextran matrix with positively charged DEAE* groups distributed throughout the matrix, is a general purpose microcarrier. It is particularly suitable for most established cell lines and for production of viruses or cell products from cultures of primary cells and normal diploid cell strains.
High yield culture of anchorage-dependent cells: Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few mL to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.
Suitable for most established cell lines: Formed by substituting a cross-linked dextran matrix with positively charged DEAE* groups distributed throughout the matrix, is a general purpose microcarrier. It is particularly suitable for most established cell lines and for production of viruses or cell products from cultures of primary cells and normal diploid cell strains.
Application
For general microcarrier culture.
The particle size of this product averages 190um when swelled in liquid solutions (as in use). The particle size of 60-87 um relates to the beads in dry form.
The particle size of this product averages 190um when swelled in liquid solutions (as in use). The particle size of 60-87 um relates to the beads in dry form.
Features and Benefits
- General-purpose microcarrier for most established, anchorage-dependent cell lines.
- Contains positive charges throughout the microporous matrix.
- Transparent for easy microscopic examination of attached cells.
Other Notes
Dextran beads with positive-charged DEAE groups throughout matrix.
Preparation Note
Prior to use, swell beads in PBS (50 mL/g) and autoclave at 120 °C.
Legal Information
Cytodex is a registered trademark of Cytiva
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Quanyu Zhao et al.
Biotechnology progress, 19(5), 1569-1573 (2003-10-04)
Toward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, and glass beads, were
Denver C Surrao et al.
Tissue engineering. Part A, 17(15-16), 1959-1967 (2011-04-01)
Tissue engineering is a promising approach for articular cartilage repair; however, it is challenging to produce adequate amounts of tissue in vitro from the limited number of cells that can be extracted from an individual. Relatively few cell expansion methods
Pierre Danhier et al.
PloS one, 8(1), e53324-e53324 (2013-02-06)
Cell detachment is a procedure routinely performed in cell culture and a necessary step in many biochemical assays including the determination of oxygen consumption rates (OCR) in vitro. In vivo, cell detachment has been shown to exert profound metabolic influences
S Mehdi Hassanzadeh et al.
Pakistan journal of biological sciences : PJBS, 14(7), 441-448 (2011-09-10)
To achieve higher titer of rabies virus higher density of host cells will need. In this study, capability of FibraCel disks packed in 500 mL spinner basket versus Cytodex-1 in 500 mL spinner flask was investigated for propagation of Vero
Christopher J Hewitt et al.
Biotechnology letters, 33(11), 2325-2335 (2011-07-20)
The effects on human mesenchymal stem cell growth of choosing either of two spinner flask impeller geometries, two microcarrier concentrations and two cell concentrations (seeding densities) were investigated. Cytodex 3 microcarriers were not damaged when held at the minimum speed
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