C0231
Anti-phospho-Cdk1 (pThr14/pTyr15) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
rat, mouse, human
technique(s)
immunoprecipitation (IP): 1:100
western blot: 1:1,000
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pThr14/pTyr15)
Gene Information
human ... CDC2(983)
mouse ... Cdc2a(12534)
General description
Cdk1 is a regulatory protein that stimulates mitosis in eukaryotic cells. Phosphorylation of Thr14 and Tyr15 residues in Cdk1 causes its inactivation, which eventually results in the exit of cells from the mitotic cycle. Cdk1 may also be in involved in protein SUMOylation during cell cycle regulation . Anti-phospho-Cdk1 (p(Thr14)/p(Tyr15)) antibody is specific for rat, human and mouse Cdk1 (p(Thr14)/p(Tyr15)) and other cyclin-dependent kinases that are phosphorylated on these amino acid residues. The antibody does not bind with Cdk6 or Cdk4.
Immunogen
synthetic phosphopeptide derived from the region of the human cdk (cyclin-dependent kinases) that contains threonine 14 and tyrosine 15.
Application
Anti-phospho-Cdk1 (p(Thr14)/p(Tyr15)) antibody is suitable for use in western blot (1:1,000), immunoblot (35kDa) and immunoprecipitation (1:100).
Physical form
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), containing 50% glycerol with 1.0 mg/mL bovine serum albumin and 0.05% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
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Yan Ma et al.
PloS one, 7(3), e33835-e33835 (2012-04-06)
Mitotic progression in eukaryotic cells depends upon the activation of cyclin-dependent kinase 1 (CDK1), followed by its inactivation through the anaphase-promoting complex (APC)/cyclosome-mediated degradation of M-phase cyclins. Previous work revealed that expression of a constitutively active CDK1 (CDK1AF) in HeLa
Yee-Fun Su et al.
PloS one, 7(4), e34250-e34250 (2012-04-18)
Increasing evidence has pointed to an important role of SUMOylation in cell cycle regulation, especially for M phase. In the current studies, we have obtained evidence through in vitro studies that the master M phase regulator CDK1/cyclin B kinase phosphorylates
S Atherton-Fessler et al.
Molecular and cellular biology, 13(3), 1675-1685 (1993-03-01)
The kinase activity of human p34cdc2 is negatively regulated by phosphorylation at Thr-14 and Tyr-15. These residues lie within the putative nucleotide binding domain of p34cdc2. It has been proposed that phosphorylation within this motif ablates the binding of ATP
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