Listed below are possible products that can be used as Square-well block plates with Product BLD9604, GenElute™ 96 Well Blood Genomic DNA. These are not provided with the kit. Product Z671371, Whatman® UNIPLATE microplates (Whatman, 7701-5200)Product CLS3960, Corning® 96 well storage system, sterile (Corning, 3960)Product CLS3961, Corning® 96 well storage system, non-sterile (Corning, 3961)
BLD9604
GenElute™ 96 Well Blood Genomic DNA Purification Kit
4 x 96 well plate, sufficient for 4 96-well plate purifications
Synonym(s):
High Throughput Purification, Blood Genomic DNA
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About This Item
UNSPSC Code:
12352200
usage
sufficient for 4 96-well plate purifications
quality
4 x 96 well plate
storage temp.
15-25°C
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General description
GenElute 96 Well Blood Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from whole blood, buffy coat, or cultured cells. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations and hazardous compounds such as phenol and chloroform.
The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove contaminants, the pure genomic DNA is eluted in the Blood Elution Solution included in the kit.
The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.
The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove contaminants, the pure genomic DNA is eluted in the Blood Elution Solution included in the kit.
The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.
Application
The purified genomic DNA is ready for use in downstream applications such as:
- PCR
- restriction digest
- Southern blots
- cloning
- enzymatic reactions
Features and Benefits
- Starting material: 200 μl fresh or frozen blood
- Expected yield: 4 - 6 μg/well
- Elution volume: 50 - 200 μl
- Time required: 70 min/plate
- Innovative wash plate minimizes risk of cross-contamination
- Reduced risk of clogging
- Complete processing possible at room temperature
Principle
The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove contaminants, the pure genomic DNA is eluted in the Blood Elution Solution included in the kit.
Other Notes
Please place blood binding plate at 4°C once you receive the kit. Other contents of the kit can be stored at room temp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Product No.
Description
Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
73.4 °F
flash_point_c
23 °C
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What can be used as the "Square-well block" plate for the centrifugation protocol for Product BLD9604, GenElute™ 96 Well Blood Genomic DNA Purification Kit?
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