BLD9601

GenElute^™ 96 Well Blood Genomic DNA Purification Kit

1 x 96 well plate, sufficient for 1 96-well plate purifications

• Synonym(s): High Throughput Purification, Blood Genomic DNA
• UNSPSC Code: 12352200

Properties

• usage: sufficient for 1 96-well plate purifications
• quality: 1 x 96 well plate
• Storage temp.: 15-25°C

Description

General description

GenElute 96 Well Blood Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from whole blood, buffy coat, or cultured cells. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations and hazardous compounds such as phenol and chloroform.

The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove contaminants, the pure genomic DNA is eluted in the Blood Elution Solution included in the kit.

The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.

Application

The purified genomic DNA is ready for use in downstream applications such as:

• PCR
• restriction digest
• Southern blots
• cloning
• enzymatic reactions

Features and Benefits

• Starting material: 200 μl fresh or frozen blood
• Expected yield: 4 - 6 μg/well
• Elution volume: 50 - 200 μl
• Time required: 70 min/plate
• Innovative wash plate minimizes risk of cross-contamination
• Reduced risk of clogging
• Complete processing possible at room temperature

Principle

The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove contaminants, the pure genomic DNA is eluted in the Blood Elution Solution included in the kit.

Other Notes

Please place blood binding plate at 4°C once you receive the kit. Other contents of the kit can be stored at room temp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS02,GHS08,GHS07
• signalword: Danger
• hcodes: H226,H302 + H332,H315,H319,H334,H335,H336
• pcodes: P210 - P233 - P240 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system, Respiratory system
• Storage Class: 3 - Flammable liquids
• flash_point_f: 73.4 °F
• flash_point_c: 23 °C