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Key Documents

B9780

Sigma-Aldrich

Monoclonal Anti-Bovine IgG−Biotin antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Bovine IgG

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About This Item

UNSPSC Code:
12352203

biological source

mouse

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

BG-18, monoclonal

form

buffered aqueous solution

species reactivity

bovine (non-reactive to IgM)

should not react with

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

technique(s)

direct ELISA: 1:50,000
dot blot: 1:60,000-1:120,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

Specificity

bovine IgG1 and IgG2

Application

Monoclonal Anti-Bovine IgG-Biotin antibody produced in mouse may be used for ELISA at a working dilution of 1:50,000. It may be suitable for dot blot at a dilution range of 1:60,000-1:120,000. The antibody was used in ELISA to determine the antibody response to rabies vaccine in cattle.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Bovine IgG antibody allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
120M4800
128K4766
116K4848
045K4751
060K4886

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J Phillips et al.
Cytotherapy, 3(3), 233-242 (2002-08-13)
CAMPATH-1 Abs have been used for T-cell depletion in stem-cell transplantation since the early 1980s. During that time there has been substantial progress in manufacturing techniques and quality control procedures. This article summarizes the methods used to produce the Abs
A C Rodrigues da Silva et al.
Revista do Instituto de Medicina Tropical de Sao Paulo, 42(2), 95-98 (2000-05-16)
Despite the absence of current official reports showing the number of cattle infected by rabies, it is estimated that nearly 30,000 bovines are lost each year in Brazil. In order to minimize the important economic losses, control of the disease
S Hashira et al.
Pediatrics international : official journal of the Japan Pediatric Society, 42(4), 337-342 (2000-09-15)
Maternal immunoglobulin G (IgG), transferred across the placenta to the fetus during intrauterine life, is an important component of the neonatal immunological defence mechanisms against infection. There is controversy with respect to differences in placental transfer of the different IgG

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