immunoprecipitation (IP): 2-5 μg using MDCK cell lysate indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed MDCK cells western blot: 0.5-1 μg/mL using lysates of MDCK cells
synthetic peptide corresponding to amino acids 275-289 of human Par6β conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Developmental dynamics : an official publication of the American Association of Anatomists, 245(6), 653-666 (2016-03-19)
TMEM47 is the vertebrate orthologue of C. elegans VAB-9, a tetraspan adherens junction protein in the PMP22/EMP/Claudin family of proteins. VAB-9 regulates cell morphology and adhesion in C. elegans and TMEM47 is expressed during kidney development and regulates the activity
Spermatogenesis is a complex process involving the regulation of multiple cell types. As the only somatic cell type in the seminiferous tubules, Sertoli cells are essential for spermatogenesis throughout the spermatogenic cycle. The Wilms tumor gene, Wt1, is specifically expressed
Partitioning defective (Par) proteins regulate cell polarity and differentiation. Par3, Par6β, and protein kinase Cζ (PKCζ), which are PAR complex members, have been shown to be associated with oncogenesis and progression. Herein, we report the expression pattern and clinical relevance
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