immunoprecipitation (IP): suitable indirect immunofluorescence: suitable western blot: suitable
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
BAPTA is a highly selective calcium chelator. BAPTA and its analog BAPTA/AM have been extensively used in cell physiology research for defining the mechanisms underlying inter- or intracellular calcium homeostasis and calcium dependent exocytosis. They may also be used in the study of diseases in which the regulation of excitatory neurotransmitter release and uptake has been disturbed.
Immunogen
BAPTA conjugated to KLH.
Application
Anti-BAPTA antibody produced in rabbit is suitable for immunoblotting, immunofluorescence or immunoprecipitation at 1-10 μg/mL. The antibody is used in determining spatial distribution of BAPTA through immunocytochemistry and confocal microscopy of cortical neurons loaded with BAPTA/AM, an analog. It is also useful in determining kinetics of loading and efflux of BAPTA through ELISAs and immunocytochemistry.
Physical form
Solution in phosphate buffered saline, pH 7.4, with 0.08% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The role of calcium in cortical granule exocytosis and activation of the cell cycle at fertilization was examined in the mouse egg using the calcium chelator BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid) and the fluorescent calcium indicator fluo-3. BAPTA and fluo-3 were introduced
The potential importance of intracellular calcium-binding proteins in rapid and highly localized Ca2+ signalling is poorly understood. During fast synaptic transmission, which occurs at specialized active zones where Ca2+ diffuses only a few tens of nanometers from channels to neurotransmitter
In spite of its importance as a tool to manipulate cell calcium, the versatility of the octadentate chelator BAPTA in cell physiological and diverse other applications is limited by the difficulty with which it can be quantified and its cell
Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications
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