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B3685

Sigma-Aldrich

Monoclonal Anti-BOB78 antibody produced in mouse

clone BOB78, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
MFCD06798697
UNSPSC Code:
12352203

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BOB78, monoclonal

form

buffered aqueous solution

mol wt

antigen ~90 kDa

species reactivity

mouse, human, monkey

concentration

~1 mg/mL

technique(s)

dot blot: suitable
flow cytometry: 1-2 μg/mL using 1x106 THP-1 cells treated with 3 μM camptothecin
immunocytochemistry: suitable
western blot: suitable

UniProt accession no.

Q9H251

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CDH23(64072)
mouse ... Cdh23(22295)

General description

The clearance of neutrophils that appear in large numbers at the inflammatory site is carried out by programmed cell death (apoptosis). An apoptotic neutrophil undergoes several changes in the amount of protein, carbohydrates and lipids. These changes are recognized by macrophages, which thus destroy the neutrophils by phagocytosis. BOB78, with a molecular mass of 90 kDa, is an up-regulated protein that appears in the granular compartment of the late apoptotic neutrophil. This protein does not appear in freshly isolated erythrocytes, platelets, lymphocytes, monocytes, or neutrophils.

Immunogen

serum-deprived THP-1 cells.

Application

Monoclonal Anti-BOB78 antibody produced in mouse is suitable for use in several immunochemical techniques such as immunoblotting, immunocytochemistry, dot-blot and flow cytometry. Monoclonal antibodies specific for BOB78 are useful for studying the biology of apoptotic neutrophils.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
064K4821

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A G Lowrie et al.
British journal of cancer, 94(11), 1663-1671 (2006-05-11)
Proteolysis-inducing factor, a cachexia-inducing tumour product, is an N-glycosylated peptide with homology to the unglycosylated neuronal survival peptide Y-P30 and a predicted product of the dermcidin gene, a pro-survival oncogene in breast cancer. We aimed to investigate whether dermcidin is
S P Hart et al.
Cell death and differentiation, 7(5), 493-503 (2000-05-09)
We have used a panel of monoclonal antibodies and lectins to examine the profile of surface molecule expression on human neutrophils that have undergone spontaneous apoptosis during in vitro culture. Neutrophil apoptosis was found to be accompanied by down-regulation of

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