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Key Documents

AV37320

Sigma-Aldrich

Anti-PYCARD antibody produced in rabbit

IgG fraction of antiserum

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About This Item

MDL number:
MFCD03096627
UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

21 kDa

species reactivity

human, mouse

concentration

0.5 mg - 1 mg/mL

technique(s)

western blot: suitable

NCBI accession no.

NP_075747

UniProt accession no.

Q9EPB4

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... PYCARD(66824)

General description

Apoptosis-associated speck-like protein containing a CARD (Pycard) is a member of a superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspases. It is found in the cytoplasm but following apoptosis it is found localized near the nucleus in ball like structures.

Immunogen

Synthetic peptide directed towards the middle region of mouse PYCARD

Application

HS5 myeloid cell lysates were subjected to western blot analysis where blots were probed with rabbit anti-pycard at a 1:1000 dilution.

Biochem/physiol Actions

Pycard is a cytosolic soluble protein that forms insoluble aggregates and enhances etoposide-induced apoptosis.

Sequence

Synthetic peptide located within the following region: TVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQAL

Physical form

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
QC6369

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Xiang Li et al.
Blood, 116(13), 2304-2314 (2010-06-22)
Patients with low-grade myelodysplastic syndromes (MDS) show high levels of tumor necrosis factor α (TNFα) and up-regulation of apoptosis in the marrow. In contrast, marrow cells in advanced MDS are typically resistant to TNFα-induced apoptosis but are rendered apoptosis-sensitive on

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