Application
The S1-10 Universal Detection Reagent is provided as a ready-to-use solution for use with the In Vitro "Fold ′n′ Glow" Solubility Assay Kits.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Stéphanie Cabantous et al.
Nature biotechnology, 23(1), 102-107 (2004-12-08)
Existing protein tagging and detection methods are powerful but have drawbacks. Split protein tags can perturb protein solubility or may not work in living cells. Green fluorescent protein (GFP) fusions can misfold or exhibit altered processing. Fluorogenic biarsenical FLaSH or
G S Waldo et al.
Nature biotechnology, 17(7), 691-695 (1999-07-15)
Formation of the chromophore of green fluorescent protein (GFP) depends on the correct folding of the protein. We constructed a "folding reporter" vector, in which a test protein is expressed as an N-terminal fusion with GFP. Using a test panel
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