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A9356

Sigma-Aldrich

Anti-ATG10 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-APG10, Anti-APG10 autophagy 10-like, Anti-APG10L, Anti-Autophagy related 10 homolog (S. cerevisiae)

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~27 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using whole extract of HEK-293T cells expressing human Atg10
western blot: 3-6 μg/mL using whole extract of HEK-293T cells expressing human Atg10

UniProt accession no.

Q9H0Y0

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ATG10(83734)

General description

ATG10 is an autophagy-related protein that functions as an E2-like enzyme to promote the addition of ubiquitination modifications essential for autophagosome formation.
Rabbit Anti-ATG10 antibody recognizes human Atg10. Detection of the Atg10 band by immunoblotting is specifically inhibited by the immunizing peptide.

Application

Rabbit Anti-ATG10 antibody can be used for immunoprecipitation (5-10μg) and western blot (3-6μg/ml) assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
058K4787

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Yuan-Yuan Ma et al.
Cell death & disease, 11(3), 200-200 (2020-03-25)
Interferon lambda-2 (IL28A) has a wide antiviral effect with fewer side-effects. Autophagy is a host mechanism to maintain intracellular homeostasis and defends invasion of pathogenic microorganisms. HCV NS5A can disable host defense systems to support HCV replication. Thus, molecular mechanism
Isei Tanida et al.
Methods in molecular biology (Clifton, N.J.), 648, 193-214 (2010-08-12)
Two major proteolysis systems, the ubiquitin-proteasome system, and the autophagy-lysosome system, contribute to degradation of various types of protein and/or protein aggregates. In general, the autophagy-lysosome system is involved in bulk intracellular degradation of proteins and organelles, while the ubiquitin-proteasome
Qiong Zhao et al.
Scientific reports, 7(1), 11250-11250 (2017-09-14)
Autophagy and immune response are two defense systems that human-body uses against viral infection. Previous studies documented that some viral mechanisms circumvented host immunity mechanisms and hijacked autophagy for its replication and survival. Here, we focus on interactions between autophagy
Liz Mariely Garcia-Peterson et al.
Genes & cancer, 8(9-10), 701-712 (2017-12-14)
Melanoma is an aggressive skin cancer that can rapidly metastasize to become fatal, if not diagnosed early. Despite recent therapeutic advances, management of melanoma remains difficult. Therefore, novel molecular targets and strategies are required to manage this neoplasm. This study
Qian Li et al.
Autophagy, 11(8), 1293-1307 (2015-07-08)
Burkholderia pseudomallei is the causative agent of melioidosis, a disease with high mortality, which is prevalent in tropical regions of the world. A recent study shows that B. pseudomallei can survive inside mammalian cells because of its ability to actively

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