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A6610

Sigma-Aldrich

Anti-ASNS (506-520) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Asparagine synthetase, Anti-TS11

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~64 kDa

species reactivity

human

technique(s)

western blot: 1:500-1:2,000

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ASNS(440)

General description

ASNS is an enzyme that promotes the production of asparagine from aspartate.
Asparagine synthetase (ASNS) deficiencies have been linked to progressive encephalopathy and congenital microcephaly. Furthermore, ASNS has also been analyzed for its role in drug-resistant childhood acute lymphoblastic leukemia (ALL). Rabbit Anti-ASNS (506-520) binds to human ASNS (506-520).

Immunogen

synthetic peptide corresponding to amino acids 506-520 of human ASNS

Application

Rabbit Anti-ASNS (506-520) antibody has been used for western blot applications at dilutions of 1:500-1:2,000.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Nigel G J Richards et al.
Annual review of biochemistry, 75, 629-654 (2006-06-08)
Modern clinical treatments of childhood acute lymphoblastic leukemia (ALL) employ enzyme-based methods for depletion of blood asparagine in combination with standard chemotherapeutic agents. Significant side effects can arise in these protocols and, in many cases, patients develop drug-resistant forms of
Philip L Lorenzi et al.
Drug news & perspectives, 22(1), 61-64 (2009-02-12)
L-Asparaginase (L-ASP) is an enzyme drug that has been an asset to leukemia treatment regimens for four decades. Variability in its clinical efficacy, however, has prompted the search for biomarkers capable of distinguishing responders from non-responders. In that regard, the
Elizabeth K Ruzzo et al.
Neuron, 80(2), 429-441 (2013-10-22)
We analyzed four families that presented with a similar condition characterized by congenital microcephaly, intellectual disability, progressive cerebral atrophy, and intractable seizures. We show that recessive mutations in the ASNS gene are responsible for this syndrome. Two of the identified

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