ADAMTS-3 belongs to the ADAM (A Disintegrin And Metalloproteinase) family and has thrombospondin (TS) repeats. Studies have suggested that ADAMTS-3 is preferably expressed in the cartilages and has procollagen II N-propeptidase functions. Rabbit Anti-ADAMTS-3, C-Terminal antibody binds to the C-terminus of human ADAMTS-3.
By immunoblotting against the reduced protein, the antibody identifies the zymogen form at 130-140 kDa, activated forms at 94-105 kDa (major bands), and breakdown products at 50 kDa, 34 kDa in cell culture media and lysates.
Immunogen
synthetic peptide corresponding to the C-terminus of human ADAMTS-3.
Application
Rabbit Anti-ADAMTS-3, C-Terminal antibody can be used for western blot applications at a dilution of 1:1,000.
Physical form
Solution in phosphate buffered saline containing 50% glycerol and 0.05% sodium azide.
The Journal of biological chemistry, 276(34), 31502-31509 (2001-06-16)
The amino and carboxyl propeptides of procollagens I and II are removed by specific enzymes as a prerequisite for fibril assembly. Null mutations in procollagen I N-propeptidase (ADAMTS-2) cause dermatosparaxis in cattle and the Ehlers-Danlos syndrome (dermatosparactic type) in humans
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