A5719
Anti-FITC-Alkaline Phosphatase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
biological source
rabbit
conjugate
alkaline phosphatase conjugate
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
dot blot: 1:2,000 using fluoresceinated nucleic acid probes
isotype
IgG
shipped in
wet ice
storage temp.
2-8°C
General description
FITC (fluorescein isothiocyanate) is a fluorophore that absorbs ultraviolet or blue light and emits visible yellow-green light. Fluorescent labeling allows quick and precise localization of antigen-antibody associations in tissues and cells. FITC is a widely used antibody marker in immunofluorescence assays, as protein-FITC conjugation is comparatively a simple task and usually does not interfere with protein function. FITC is also utilized as a hapten to label proteins. Thus, anti-FITC antibodies can facilitate the analyses of FITC labeled proteins and antibody responses to haptens.
Immunogen
FITC conjugated to BSA
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Immunohistochemistry of paraffin embedded, 2% paraformaldhyde fixed adult rectal sections was performed using alkaline phosphatase-conjugated rabbit anti-FITC antibody (A4843) at a 1:100 dilution.
Rabbit Anti-FITC-Alkaline Phosphatase antibody has been used to detect CFSE labelled cells using immunohistochemical techniques. The antibody can also be used for dot blot (1:2,000 using fluorinated nucleic acid probes).
The conjugate is formulated to detect FITC-labeled, nucleic acid probes in membrane-based blotting applications.
Caution
Do not freeze.
Physical form
Solution in Tris-buffered saline, pH 8.0, containing 1% bovine serum albumin and 0.1% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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James B Chung et al.
International immunology, 14(2), 157-166 (2002-01-26)
Transitional immature B cells undergo apoptosis and fail to proliferate in response to BCR cross-linking, thus representing a target for negative selection of potentially autoreactive B cells in vivo. In agreement with recent reports, transitional B cells were divided into
Romana Illig et al.
RNA (New York, N.Y.), 15(8), 1588-1596 (2009-06-25)
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