A5680
Monoclonal Anti-Avidin antibody produced in mouse
clone WC19.10, ascites fluid
Synonym(s):
Monoclonal Anti-Avidin
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
WC19.10, monoclonal
contains
15 mM sodium azide
technique(s)
immunohistochemistry (frozen sections): suitable
indirect ELISA: 1:2,000
western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.
Immunogen
Purified egg white avidin.
Application
Immunohistochemistry was performed on 4% paraformaldhyde fixed mouse brain sections. After incubation with primary antibody, the sections were treated with a biotinylated secondary antibody and the with texas-red conjugated egg white avidin. The signal was then amplified by treating samples with mouse monoclonal anti-avidin (A5680) at a dilution of 1:1000.
Mouse Monoclonal Anti-Avidin antibody has been used for ELISA assays at 3-5mg/ml. The antibody has also been used for immunocytochemistry at 1:2000 or 1:4000 dilutions.
Physical form
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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D M Kennedy et al.
Clinical and experimental immunology, 98(2), 245-251 (1994-11-01)
As elevated levels of glycated IgG have been detected in the plasma of patients with diabetes mellitus, a disease associated with increased susceptibility to infection, we have investigated whether glycation of MoAbs affects the kinetics and/or affinity of antigen binding.
Willem Kamphuis et al.
Methods in molecular biology (Clifton, N.J.), 418, 129-138 (2008-02-22)
Immunodetection techniques are dependent on enzyme-protein conjugates for the visualization of antigen-antibody complexes. One of the most widely used is the avidin-biotin-peroxidase complex (ABC) method. However, treatment of certain tissues with ABC reagents alone may result in high background, which
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