A4720
Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse
clone PY20, purified immunoglobulin, buffered aqueous suspension
Synonym(s):
Monoclonal Anti-Phosphotyrosine, Phospho-Tyr, Phospho-tyrosine, p-Tyr
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About This Item
UNSPSC Code:
12352203
biological source
mouse
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PY20, monoclonal
form
buffered aqueous suspension
technique(s)
immunoprecipitation (IP): suitable
isotype
IgG2b
shipped in
wet ice
storage temp.
2-8°C
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General description
Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine−agarose antibody can be used in immunoprecipitation. Mouse anti-phosphotyrosine−agarose antibody reacts specifically with both native and denatured proteins comprising phosphorylated tyrosine.
Immunogen
phosphotyrosine-protein conjugate
Application
Monoclonal anti-phosphotyrosine−agarose antibody can be used in immunoaffinity purification of phosphotyrosyl proteins, including transforming proteins, steroid hormone receptors (e.g., estradiol and glucocorticoid receptors), growth factor receptors (e.g., EGF and PDGF receptors), insulin receptors and other proteins involved in the control of cellular growth.
Physical form
Suspension in 20 mM sodium phosphate, pH 7.5, 150 mM sodium chloride, 3 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
pcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Vanessa Liévin-Le Moal et al.
Cellular microbiology, 13(5), 764-785 (2011-03-01)
CEACAM1 expressed by granulocytes and epithelial cells is recognized as a membrane-associated receptor by some Gram-negative pathogens. Here we report a previously unsuspected role of human CEACAM1-4L (hCEACAM1-4L) in polarized epithelial cells. We find that in contrast with non-transfected cells
A Dvir et al.
The Journal of cell biology, 113(4), 857-865 (1991-05-01)
Protein tyrosine kinase blockers of the tyrphostin family inhibited the EGF-dependent proliferation of human and guinea pig keratinocytes grown in culture and induced their growth arrest. These blockers also significantly inhibited the growth of epidermal keratinocytes, but not of dermal
Wiljan J A J Hendriks et al.
The FEBS journal, 275(5), 816-830 (2008-02-27)
Some 40-odd genes in mammals encode phosphotyrosine-specific, 'classical' protein tyrosine phosphatases. The generation of animal model systems and the study of various human disease states have begun to elucidate the important and diverse roles of protein tyrosine phosphatases in cellular
Joanna Cieśla et al.
Acta biochimica Polonica, 58(2), 137-148 (2011-05-31)
Reversible phosphorylation is the most widespread posttranslational protein modification, playing regulatory role in almost every aspect of cell life. The majority of protein phosphorylation research has been focused on serine, threonine and tyrosine that form acid-stable phosphomonoesters. However, protein histidine
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