A4095

Anti-phospho-ATF-2 (pThr^69,71) antibody, Mouse monoclonal

clone ATF-22P, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-phospho-ATF-2 (pThr^69,71)
• MDL number: MFCD03096614
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ATF-22P, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 60-70 kDa (triplet)
• species reactivity: frog, mouse, rat, chicken, human
• concentration: 0.5-1.0 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P15336
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pThr69/pThr71)
• Gene Information: human ... ATF2(1386) ; mouse ... Atf2(11909) ; rat ... Atf2(81647)

Description

General description

ATF2 (Activating Transcription Factor 2) is a member of the leucine zipper family of transcription factors. It binds to CRE domains and stimulates CRE-dependent transcription.

Mouse monoclonal anti-phospho-ATF-2 (pThr^69,71) reacts specifically with ATF2 phosphorylated at both threonine 69 and 71, and does not detect the monophosphorylated and the non-phosphorylated ATF2 molecule. The epitope recognized by the antibody resides in the N-terminal region of ATF2 (aa 66-79, pT^69,71). This epitope is identical in ATF2 of human, rat, mouse, chicken and frog. The antibody cross-reacts with human, rat and mouse ATF2.

Immunogen

synthetic peptide corresponding to the N-terminal amino acids 66-79 (pT^69,71) of human ATF2. This sequence is identical in ATF2 from human, rat, mouse, chicken, and frog.

Application

Mouse monoclonal anti-phospho-ATF-2 (pThr^69,71) antibody can be used for immunocytochemistry, indirect ELISA, microarray, and western blot.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable