This product is soluble in water up to 50 mg/mL. The appropriate stock concentration for assays can be determined by the end user. The molecular weight is 551.14 (anhydrous basis), and the water content can be found on the lot-specific certificate of analysis. Using the molecular weight, the water content, and the chosen concentration for the ATP solution, the molarity can be calculated.
A2383
Adenosine 5′-triphosphate disodium salt hydrate
Adenosine 5′-triphosphate disodium salt hydrate
Grade I, ≥99%, from microbial
Synonym(s):
ATP disodium salt
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1 G
$79.40
5 G
$194.00
10 G
$297.00
25 G
$698.00
$79.40
Available to ship onApril 29, 2025Details
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1 G
$79.40
5 G
$194.00
10 G
$297.00
25 G
$698.00
About This Item
Empirical Formula (Hill Notation):
C10H14N5Na2O13P3 · xH2O
CAS Number:
Molecular Weight:
551.14 (anhydrous basis)
EC Number:
MDL number:
UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
NACRES:
NA.51
$79.40
Available to ship onApril 29, 2025Details
Recommended Products
biological source
microbial
Quality Level
type
Grade I
assay
≥99%
form
powder
solubility
H2O: 50 mg/mL
storage temp.
−20°C
SMILES string
O.[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
InChI
1S/C10H16N5O13P3.2Na.H2O/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;;1H2/q;2*+1;/p-2/t4-,6-,7-,10-;;;/m1.../s1
InChI key
NTBQNWBHIXNPRU-MSQVLRTGSA-L
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Application
Adenosine 5′-triphosphate disodium salt hydrate has been used:
- to prepare adenosine triphosphate (ATP) standard solutions to determine ATP levels in various bacterial cultures[1]
- bone marrow-derived macrophages (BMDM) treatment[2]
- as a preparation free of contaminating guanine nucleotides to study the binding effects of coatomer β subunit (β-COP)[3]
Biochem/physiol Actions
Adenosine 5′-triphosphate (ATP) is a central component of energy storage and metabolism in vivo.[4] ATP is used in many cellular processes, respiration, biosynthetic reactions, active transport, and motility.[5] ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase that produce the secondary messenger cyclic adenosine monophosphate (cAMP).[6] ATP provides the metabolic energy to drive metabolic pumps.[5] It serves as a coenzyme in a wide array of enzymatic reactions.[7]
P2 purinergic agonist; increases activity of Ca2+-activated K+ channels; substrate for ATP-dependent enzyme systems
P2 purinocepter agonist.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type N95 (US)
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Customers Also Viewed
Release of extracellular ATP by bacteria during growth
Mempin R, et al.
BMC Microbiology, 13(1), 301-301 (2013)
J G Donaldson et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(14), 6408-6412 (1992-07-15)
The coatomer is a cytosolic protein complex that reversibly associates with Golgi membranes and is implicated in modulating Golgi membrane transport. The association of beta-COP, a component of coatomer, with Golgi membranes is enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[gamma S]), a
Rui Nian et al.
Biotechnology progress, 25(4), 1078-1085 (2009-06-25)
The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone-mediated refolding of heat-denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the
Mass spectrometry and NMR spectroscopy based quantitative metabolomics
Proteomics. Clinical Applications (2020)
Physiology, Adenosine Triphosphate (ATP)
NCBI Bookshelf null
Protocols
Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.
Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)
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What volume of water should 1mg ATP be reconstituted in & what would be its corresponding concentration?
1 answer-
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Can I dissolve this ATP in water and store it?
1 answer-
This item can be solubilized in water at 50 mg/mL yielding a clear, colorless solution. Aqueous solutions of ATP are stable for months at -15C and or one week when stored at -0C as mentioned in the Product Information Sheet.
For more information, please navigate to the ‘DOCUMENTATION’ section to access the Product Information Sheet under 'More Documents':
https://www.sigmaaldrich.com/product/sigma/a2383#product-documentationHelpful?
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