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A1607

Sigma-Aldrich

Anti-ACTR3B (351-365) antibody produced in rabbit

IgG fraction of antiserum, PBS solution

Synonym(s):

Anti-ARP11, Anti-ARP3β, Anti-Actin-related protein 3β isoform 1

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

PBS solution

mol wt

antigen ~47 kDa

species reactivity

human

technique(s)

indirect immunofluorescence: suitable
western blot: 1:500-1:2,000

UniProt accession no.

Q9P1U1

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACTR3B(57180)

General description

ACTR3B (Arp11) is known to affect dynein-dynactin interactions and is required for dynein activities in Aspergillus nidulans. Furthermore, Arp11 can also inhibit the tumorigenicity of human lung adenocarcinoma cells (PC-14). Rabbit anti-ACTR3B (351-365) binds to human ACTR3B (351-365).

Immunogen

synthetic peptide corresponding to amino acids 351-365 of human ACTR3B.

Application

Rabbit anti-ACTR3B (351-365) can be used for western blot (1:500-1:2,000) and indirect immunofluorescence.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
116M4850V
068K4866

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Jun Zhang et al.
Traffic (Copenhagen, Denmark), 9(7), 1073-1087 (2008-04-16)
The dynactin complex contains proteins including p150 that interacts with cytoplasmic dynein and an actin-related protein Arp1 that forms a minifilament. Proteins including Arp11 and p62 locate at the pointed end of the Arp1 filament, but their biochemical functions are
Nobuko Shindo-Okada et al.
Biochemical and biophysical research communications, 312(4), 889-896 (2003-12-04)
We recently reported the isolation of a novel actin-related gene, Arp11, from a PC-14 human lung adenocarcinoma clone with low metastatic and tumorigenic ability. In this paper, we report the effect of Arp11 expression on PC-14 tumor growth. We transfected

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