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A1543

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Goat Anti-Human IgG (whole molecule)−AP

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1 ML
$427.00

$427.00

Available to ship onMay 01, 2025Details

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1 ML
$427.00

About This Item

MDL number:
MFCD00162453
UNSPSC Code:
12352203
NACRES:
NA.46

$427.00

Available to ship onMay 01, 2025Details

Request a Bulk Order

biological source

goat

Quality Level

200

recombinant

expressed in goat

conjugate

alkaline phosphatase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:20,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot (chemiluminescent): 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains, with molecular weight of 50kDa and 25kDa respectively. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Goat anti-human IgG binds to human IgG. Immunoglobulin G (IgG) is part of the immunoglobulin family and is a widely expressed serum antibody. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains.

Immunogen

IgG purified from human serum

Application

Goat anti-human IgG can also be used for dot blot (1:30,000) and immunohistochemical (1:50) applications.
Serum human IgG levels were analyzed by solid phase ELISA assays using alkaline phosphatase-conjugated goat anti-human IgG antibody incubated for 1 hour at 37°C. Assays was developed using 0.1% p-nitrophenyl phosphate (Sigma) as the substrate. Antibodies to the Seoul (strain 80/39) virus was detected in human sera by western blot analysis using alkaline phosphatase-conjugated goat anti-human IgG antibody at a 1:1000 dilution. Blots were developed using nitroblue tetrazolium and 5-bromo-4-chloro-3-indoyl-phosphate substrate (Sigma).

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000341554
0000328714
0000329427
0000245307
0000152023

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Sophana Chea et al.
Frontiers in immunology, 15, 1368066-1368066 (2024-05-16)
Aedes spp. are the most prolific mosquito vectors in the world. Found on every continent, they can effectively transmit various arboviruses, including the dengue virus which continues to cause outbreaks worldwide and is spreading into previously non-endemic areas. The lack
Antibody structure, instability, and formulation
Wei W, et al.
Journal of Pharmaceutical Sciences, 1-26 (2007)
C T Ravirajan et al.
Rheumatology (Oxford, England), 43(4), 442-447 (2004-03-17)
There is considerable evidence suggesting that anti-double-stranded deoxyribonucleic acid (anti-dsDNA) antibodies are involved in the pathogenesis of lupus nephritis. It was shown previously using severe combined immune deficient (SCID) mice that when the hybridomas secreting human immunoglobulin G (IgG) anti-dsDNA
Molouk Beiromvand et al.
Journal of infection in developing countries, 13(2), 154-161 (2020-02-10)
Cystic Echinococcosis (CE) and toxocariasis caused by the larval stages of intestinal dog worms including Echinococcus granulosus and Toxocara spp. are among the most widespread zoonotic diseases. Four hundred municipal waste collectors were serologically evaluated for CE and toxocariasis. To
L Lledó et al.
Journal of medical microbiology, 51(10), 861-865 (2002-11-19)
Data relating to hantavirus infection in Spain are scarce and limited to rural areas. The aim of this work was to study the seroprevalence of hantavirus infection in the Autonomous Community of Madrid (ACM), a region containing both rural and
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Questions

1–3 of 3 Questions  

  1. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  2. What is the source of the alkaline phosphatase that is used to make Product A1543, Anti-Human IgG (whole molecule)-Alkaline Phosphatase antibody produced in goat?

    1 answer

    1. Product A1543, Anti-Human IgG (whole molecule) -Alkaline Phosphatase antibody produced in goat, is prepared using alkaline phosphatase from bovine intestinal mucosa.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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