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A1231

Sigma-Aldrich

Anti-Aurora-A Kinase antibody, Mouse monoclonal

clone 35C1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Aurora-A Kinase, Monoclonal Anti-Aurora-A Kinase antibody produced in mouse, Anti-ARK-1, Anti-AURORA2, Anti-Aurora-related kinase 1, Anti-BTAK, Anti-STK15, Anti-STK6, Anti-Serine/threonine protein kinase 15

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About This Item

MDL number:
MFCD06411780
UNSPSC Code:
12352203
eCl@ss:
32160410
NACRES:
NA.44

biological source

mouse

recombinant

expressed in mouse cell line

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

35C1, monoclonal

form

PBS solution

mol wt

antigen ~46 kDa

species reactivity

human, mouse

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using HeLa total cell extract

isotype

IgG2b

UniProt accession no.

O14965

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AURKA(6790) , AURKA(6790)
mouse ... Aurka(20878) , Aurka(20878)

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General description

Anti-Aurora-A Kinase antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma 35C1 produced by the fusion of mouse myeloma cells (SP2/0-Ag14) and splenocytes from BALB/c mice immunized with recombinant human Aurora-A Kinase. Aurora-A Kinase is encoded by serine/threonine kinase (STK15) gene. During S-phase to mitosis progression in cell cycle, the protein is located at the centrosome.

Specificity

Monoclonal Anti-Aurora-A Kinase antibody is specific for Aurora-A Kinase in humans and mice.

Immunogen

recombinant human Aurora-A kinase.

Application

Anti-Aurora-A Kinase antibody, Mouse monoclonal has been used in immunohistochemistry.
Monoclonal Anti-Aurora-A Kinase antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot, indirect ELISA and western blot (0.25-0.5 μg/mL using HeLa total cell extract). The antibody product does not inhibit kinase activity of Aurora-A kinase, hence the product can be used to measure the in vivo aurora-A kinase activity after immunoprecipitation.

Biochem/physiol Actions

Aurora-A (Aur-A) kinase is a serine threonine kinase that regulates cell division. Elevated levels of Aur-A have been associated with inhibition of apoptosis, and evasion of spindle assembly checkpoints. Aur-A may also be used as a prognostic marker for nasopharyngeal cancers in humans .
Aurora-A kinase is involved in centrosome separation, centrosome maturation, bipolar spindle assembly and stability. Monopolar spindles are formed in the cell when the expression of aAurora-A kinase is inhibited by an appropriate siRNA, while over-expression of this protein results in centrosome amplification and polyploidy of the cell because of cytokinesis failure. Aurora-A activity is essential for the recruitment of cyclin dependent kinase (CDK)-cyclin B1 to the centrosome, which relates with its activation and the commitment of the cells to mitosis. Activation of CDK1 kinase is by dephosphorylation of Tyr15 by CDC25B. Aurora-A kinase phosphorylates CDC25B both in vitro and in vivo at Ser353. This phosphorylation occurs at the centrosome during progression from prophase to anaphase. This regulation is important for entry into mitosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Shulan Sun et al.
Cancer communications (London, England), 41(9), 851-866 (2021-07-13)
Increasing studies have reported that oncogenes regulate components of the immune system, suggesting that this is a mechanism for tumorigenesis. Aurora kinase A (AURKA), a serine/threonine kinase, is involved in cell mitosis and is essential for tumor cell proliferation, metastasis
Qian He et al.
Molecular biology of the cell, 25(11), 1715-1729 (2014-04-04)
We show here that human embryonic stem (ES) and induced pluripotent stem cell-derived neuroprogenitors (NPs) develop primary cilia. Ciliogenesis depends on the sphingolipid ceramide and its interaction with atypical PKC (aPKC), both of which distribute to the primary cilium and
Edward J Morris et al.
Nature communications, 8, 15289-15289 (2017-05-06)
Cancer cells frequently have amplified centrosomes that must be clustered together to form a bipolar mitotic spindle, and targeting centrosome clustering is considered a promising therapeutic strategy. A high-content chemical screen for inhibitors of centrosome clustering identified Stattic, a Stat3
Sisi Li et al.
Heliyon, 9(7), e17386-e17386 (2023-07-07)
The contribution of oncogenes to tumor-associated RNA splicing and the relevant molecular mechanisms therein require further elaboration. Here, we show that oncogenic Aurora kinase A (AURKA) promotes breast cancer-related RNA aberrant splicing in a context-dependent manner. AURKA regulated pan-breast cancer-associated
Aurora-A Identifies Early Recurrence and Poor Prognosis and Promises a Potential Therapeutic Target in Triple Negative Breast Cancer
Xu J, et al.
PLoS ONE, 8(2), e56919-e56919 (2013)
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