The actual amount of powder per pack will depend on the units/mg protein of the lot received. This value will be provided in the lot-specific Certificate of Analysis. For example, lot SLCN0121 tested at 1474 units/mg protein. The 100UN pack size will provide 0.06mg of protein, the 500UN pack size will provide 0.34mg of protein, and the 2500UN pack size will provide 1.70mg of protein. Please see the link below to review this Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A0521-BULKSLCN0121.pdf
A0521
α-Amylase from human saliva
Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein
Synonym(s):
1,4-α-D-Glucan-glucanohydrolase
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100 UNITS
$70.40
500 UNITS
$323.00
2500 UNITS
$535.06
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100 UNITS
$70.40
500 UNITS
$323.00
2500 UNITS
$535.06
About This Item
Recommended Products
type
Type IX-A
Quality Level
form
lyophilized powder
specific activity
1,000-3,000 units/mg protein
composition
Protein, 5.0-15.0% biuret
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
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UniProt accession no.
greener alternative category
storage temp.
−20°C
Gene Information
human ... AMY1A(276)
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General description
α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated.[1] α-amylase gene is located on human chromosome 1p21.1.[2] α-amylase is mainly produced in pancreas and saliva.[3]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
Biochem/physiol Actions
Unit Definition
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
Physical form
Lyophilized powder containing (NH4)2SO4 and sodium citrate
Preparation Note
Chromatographically purified
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
substrate
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Certificates of Analysis (COA)
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Protocols
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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Good morning, I allow myself to contact you to know the mass of the product "α-amylase from Human Saliva" (A0521-100UN). It lacks the mass of the product and I would like to know it.
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Dear Dr, how much mg saliva amylase are present in glass. We design the experimet sets. So need to know the Total amount of the amylase(mg) in the bottle. Thanks
1 answer-
The exact amount is determined by the units/mg protein listed on the Certificate of Analysis. For example, the lot SLCN0121 tested at 1474 units per milligram protein. The total amount of the amylase (mg) in the bottle for the 100 unit package contains 0.07 mg, the 500 unit package contains 0.34 mg, and the 2500 unit package will contain 1.70 mg of protein. This lot is 11.8% protein. These values will change from lot to lot, but the actual mass in the bottles remains low. Other products, such as A4551, are sold by weight. See the link below to review this product page. https://www.sigmaaldrich.com/product/sigma/a4551
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