A0438
Alcohol Oxidase from Hansenula sp.
vacuum-dried powder, ≥0.6 units/mg solid
Synonym(s):
Alcohol:oxygen oxidoreductase
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biological source
fungus (Hansenula spp.)
Quality Level
form
vacuum-dried powder
specific activity
≥0.6 units/mg solid
mol wt
~600 kDa
storage temp.
−20°C
Application
Alcohol Oxidase from Hansenula sp. has been used:
- in the immobilization on amperometric biosensor by glutaraldehyde cross linking[1]
- as a component in pre-mixed hydrogels for the electrode preparation in the biosensors[2]
- in the modification of screen printed Co-phthalocyanine/ Carbon electrode for alcohol biosensor preparation[3]
Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes [4]. It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes.
Biochem/physiol Actions
Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm [4].
Catalyzes the oxidization of primary alcohols to aldehydes.
Molecular Weight: ~600 kDa
Optimum pH: 8.5
pH Range: 5.5-8.5
Km values with the following substrates:
Allyl alcohol 33.3 mM
Benzyl alcohol 26.6 mM
Ethanol 16.2 mM
Formaldehyde 2.6 mM
Methanol 2.15 mM
N-Butanol 166 mM
n-Propanol 66 mM
Cofactor: FAD
Inhibitors: 1,4-butynediol (irreversible), propargyl alcohol (irreversible), cyclopropanol, cyclopropanone (suicide substrate), formaldehyde, H2O2, hydroxylamine, KBr, KCN, methanol (substrate inhibitor), NaN3, PCMB, propynal, urea, 4-chloromercuribenzoic acid
Molecular Weight: ~600 kDa
Optimum pH: 8.5
pH Range: 5.5-8.5
Km values with the following substrates:
Allyl alcohol 33.3 mM
Benzyl alcohol 26.6 mM
Ethanol 16.2 mM
Formaldehyde 2.6 mM
Methanol 2.15 mM
N-Butanol 166 mM
n-Propanol 66 mM
Cofactor: FAD
Inhibitors: 1,4-butynediol (irreversible), propargyl alcohol (irreversible), cyclopropanol, cyclopropanone (suicide substrate), formaldehyde, H2O2, hydroxylamine, KBr, KCN, methanol (substrate inhibitor), NaN3, PCMB, propynal, urea, 4-chloromercuribenzoic acid
Unit Definition
One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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An alcohol oxidase biosensor using PNR redox mediator at carbon film electrodes
Barsan MM and Brett CMA
Talanta, 74(5), 1505-1510 (2008)
Madalina M Barsan et al.
Talanta, 74(5), 1505-1510 (2008-03-29)
A new amperometric biosensor for ethanol monitoring has been developed and optimised. The biosensor uses poly(neutral red) (PNR), as redox mediator, which is electropolymerised on carbon film electrodes and alcohol oxidase (AlcOx) from Hansenula polymorpha as recognition element, immobilised by
B Vinet
Clinical chemistry, 33(12), 2204-2208 (1987-12-01)
This method for the specific determination of methanol in serum is based on the following two reactions: (formula; see text) Alcohol oxidase is not specific: it converts all lower alcohols to their corresponding aldehydes; however, formaldehyde dehydrogenase is specific and
Bienzyme biosensors for glucose, ethanol and putrescine built on oxidase and sweet potato peroxidase
Castillo J, et al.
Biosensors And Bioelectronics, 18(5-6), 705-714 (2003)
Hu Jin et al.
Applied biochemistry and biotechnology, 165(2), 559-571 (2011-05-14)
Improved porcine interferon-α (pIFN-α) production by recombinant Pichia pastoris was achieved by culture conditions optimization in a 5-l bioreactor. The results indicated that the pIFN-α concentration, specific methanol consumption rate, specific activities of alcohol oxidase, formaldehyde dehydrogenase, and formate dehydrogenase
Protocols
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
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