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96827

Sigma-Aldrich

Renaturation Basic Kit for Proteins

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About This Item

UNSPSC Code:
12171500

storage temp.

2-8°C

General description

The Renaturation Basic Kit for Proteins is a rapid empirical screening method used to determine the best conditions for the renaturation of proteins which have been solubilized from inclusion bodies.[1][2][3] Physical parameters, such as pH, redox potential, ionic strength, low-molecular weight additives and temperature, may have an effect on the efficiency of in vitro renaturation processes. Screening with this kit is an easy method to find renaturation conditions in a defined pH range of 6.5 to 8.5, defined redox potential and ionic strength conditions, as well as the effect of a sulfobetaine [3-(1-1-pyridinio)-1-propanesulfonate, included in the kit]. Other parameters (e.g., concentration of the solubilized protein, temperature and additional additives) can easily be added to the basic screening conditions of this kit.

Features and Benefits

Advantages
  • Comprehensive, efficient screening
  • Stringent purity standards
  • Prefabricated reagents, application sheets, and observation sheets
  • Low incidence of undesirable solubility effects

Components

The kit contains 50 components with 10 mL each. Most components can be ordered separately as 100 mL packages.

Kit Components Only

Product No.
Description
  • MES pH 6.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution
  • MES pH 6.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M solution
  • TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution
  • TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution
  • TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution
  • TRIS hydrochloride pH 8.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution
  • TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution
  • MES pH 6.5; 0.05M – Sodium chloride 0.1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – 0.1M Sodium chloride, L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • MES pH 6.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution
See All (13)

Kit Components Also Available Separately

Product No.
Description
SDS
  • MES pH 6.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution
  • 82953MES pH 6.5; 0.05M – Sodium chloride 0.2M solutionSDS
  • MES pH 6.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution
  • 73021MES pH 6.5; 0.05M – DTT 0.001M Solution solutionSDS
  • 71023MES pH 6.5; 0.05M – DTT 0.001M – PPS 1M solutionSDS
  • 83809MES pH 6.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solutionSDS
  • MES pH 6.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • MES pH 6.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M , PPS 1M solution
  • MES pH 6.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution
  • MES pH 6.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • MES pH 6.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • 94717HEPES Sodium salt pH 7.5; 0.05M solutionSDS
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – DTT 0.001M solution
  • HEPES Sodium salt pH 7.5; 0.05M – DTT 0.001M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • HEPES Sodium salt pH 7.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solution
  • HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • 80409HEPES Sodium salt pH 7.5; 0.05M – Sodium chloride 0.2M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solutionSDS
  • TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M solution
  • TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M solution
  • TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – PPS 1M solution
  • TRIS hydrochloride pH 8.5; 0.05M – DTT 0.001M solution
  • 70603TRIS hydrochloride pH 8.5; 0.05M – DTT 0.001M – PPS 1M solutionSDS
  • 81220TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – DTT 0.001M – PPS 1M solutionSDS
  • 72259TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.2M – DTT 0.001M solutionSDS
  • TRIS hydrochloride pH 8.5; 0.05M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • TRIS hydrochloride pH 8.5; 0.05M –Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M solution
  • 72403TRIS hydrochloride pH 8.5; 0.05M – Sodium chloride 0.1M – L-Glutathione red. 0.005M – L-Glutathione ox. 0.001M – PPS 1M solutionSDS
See All (36)

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCBG5546

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A Hofmann et al.
Analytical biochemistry, 230(1), 8-15 (1995-09-01)
Protein renaturation is of particular interest not only for the basic mechanisms of protein folding but also as a practical problem for proteins overexpressed in microorganisms, since recombinant proteins may accumulate as misfolded aggregates in "inclusion bodies" that are inactive
M Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(7), 3431-3435 (1997-04-01)
Large regions of genomic colinearity have been demonstrated among grass species by recombinational mapping, but the degree of chromosomal conservation at the sub-centimorgan level has not been extensively investigated. We cloned the rice and sorghum genes homologous to the sh2
R Rudolph et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 10(1), 49-56 (1996-01-01)
Insoluble, inactive inclusion bodies are frequently formed upon recombinant protein production in transformed microorganisms. These inclusion bodies, which contain the recombinant protein in an highly enriched form, can be isolated by solid/liquid separation. After solubilization, native proteins can be generated

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