93309

TRIS borate – EDTA buffer substance

for Northern and Southern blotting, powder

• Synonym(s): TBE buffer dry blend
• UNSPSC Code: 41105319
• NACRES: NA.25

Properties

• grade: Molecular Biology
• product line: BioReagent
• form: powder
• quality: 5× concentrate
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• color: white
• pH: 8.1-8.5
• storage temp.: room temp

Description

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble and protected from degradation. This powder should be reconstituted in molecular biology grade water before use.

Application

TRIS borate – EDTA buffer substance is suitable for use as running buffer for agarose gel electrophoresis[1] and nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Components

The final 5x TBE liquid contains 0.445M TRIS borate (pH 8.3) and 0.01M EDTA in water.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H360FD
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges