93145
1,3,5-Triphenyltetrazolium formazan
≥90% (UV)
Synonym(s):
TTC formazan
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About This Item
Empirical Formula (Hill Notation):
C19H16N4
CAS Number:
Molecular Weight:
300.36
Beilstein/REAXYS Number:
753057
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
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assay
≥90% (UV)
form
powder
solubility
dioxane: 50 mg/mL, clear
SMILES string
N(\N=C(/N=N/c1ccccc1)c2ccccc2)c3ccccc3
InChI
1S/C19H16N4/c1-4-10-16(11-5-1)19(22-20-17-12-6-2-7-13-17)23-21-18-14-8-3-9-15-18/h1-15,20H/b22-19-,23-21+
InChI key
BEIHVSJTPTXQGB-YMGPPEANSA-N
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General description
1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.[1]
Application
1,3,5-Triphenyltetrazolium formazan has been used:
- for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells[1]
- as a standard to study the optical density of formazan produced by TTC-treated milled seeds[2]
- to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry[3]
Other Notes
Review: Tetrazolium salts and formazans[4]
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Quantification of triphenyl-2H-tetrazoliumchloride reduction activity in bacterial cells
Defez R, et al.
Bio-protocol, 7(2), e2115-e2115 (2017)
Se-Chan Kim et al.
Journal of visualized experiments : JoVE, (65), e3896-e3896 (2012-08-01)
Surgical trauma by thoracotomy in open-chest models of coronary ligation induces an immune response which modifies different mechanisms involved in ischemia and reperfusion. Immune response includes cytokine expression and release or secretion of endogenous ligands of innate immune receptors. Activation
Laura Lopez Del Egido et al.
Frontiers in plant science, 8, 747-747 (2017-06-01)
A comparative analysis was carried out of published methods to assess seed viability using 2,3,5-triphenyltetrazolium chloride (TTC) based assays of seed batches. The tests were carried out on seeds of barley (Hordeum vulgare cv. Optic) as a model. We established
F.P. Altman
Progress in Histochemistry and Cytochemistry, 9(3), 51-51 (1976)
Roberto Defez et al.
Bio-protocol, 7(2), e2115-e2115 (2017-01-20)
This protocol describes the use of the 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt to evaluate the cell redox potential of rhizobia cells. The production of brightly colored and insoluble 1,3,5-Triphenyltetrazolium formazan arising from TTC reduction is irreversible and can be easily quantified
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