Galactose-6-phosphate is produced by the hydrolysis of lactose-phosphate by phospho-beta-galactosidase and feeds into the tagatose pathway afters its isomerization to tagatose-6-phosphate. Intracellular accumulation D-Galactose 6-Phosphate in bacteria triggers induction of the lactose operon resulting in galactose catabolism.
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Bottomless glass bottle. Contents are inside inserted fused cone.
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Journal of bacteriology, 154(3), 1204-1214 (1983-06-01)
Cells of Lactobacillus casei grown in media containing galactose or a metabolizable beta-galactoside (lactose, lactulose, or arabinosyl-beta-D-galactoside) were induced for a galactose-phosphoenolpyruvate-dependent phosphotransferase system (gal-PTS). This high-affinity system (Km for galactose, 11 microM) was inducible in eight strains examined, which
Several amino acids in the active center of the 6-phospho-beta-galactosidase from Lactococcus lactis were replaced by the corresponding residues in homologous enzymes of glycosidase family 1 with different specificities. Three mutants, W429A, K435V/Y437F and S428D/ K435V/Y437F, were constructed. W429A was
Binding of carbohydrate ligand by human C-reactive protein (CRP), in both native form and structurally deviated form (neoCRP or mCRP), was investigated using galactose-6-phosphate (Gal6P)- and Galbeta3GalNAc-containing bovine serum albumin (BSA) derivatives. To this end, we synthesized glycosides of Gal6P
Journal of molecular biology, 269(5), 851-860 (1997-06-27)
The initial structural model of 6-phospho-beta-galactosidase from Lactococcus lactis was refined to an R-factor of 16.4% (R[free] = 23.6%) to 2.3 A resolution (1 A = 0.1 nm), and the structures of three other crystal forms were solved by molecular
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