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89149

Sigma-Aldrich

Fluorescent IEF-Marker pI 5.2 solution

1 mg/mL in 5 mM HCl

Synonym(s):

IEF-Marker fluorescent pI 5.2

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About This Item

UNSPSC Code:
41105336
NACRES:
NA.26

form

liquid

mol wt

Mw 285.30 g/mol

concentration

1 mg/mL in 5 mM HCl

fluorescence

λex 336 nm; λem 424 nm (in 0.1 M citrate pH 5.2)

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

storage temp.

2-8°C

General description

filtered through a 0.45 μm membrane filter

Application

suitable as IEF-Marker for HPCE and for IEF-gels

Reconstitution

For use in HPCE the marker should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the solution can be loaded directly onto the gel (max. 1 μl per lane).

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificates of Analysis (COA)

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Articles

Isoelectric Focusing

Information on Isoelectric Focusing including what it is and how it is used. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed.

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